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Arginine uptake and metabolism in cultured murine macrophages

  • General Inflammation
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Abstract

Transport ofl-arginine and nitrite production were examined in the murine macrophage cell line J774. Lipopolysaccharide (LPS) induced a dose- and time-dependent stimulation of nitrite production which was further increased in the presence of interferon-γ (INF-γ). Nitrite synthesis was dependent on extracellular arginine and inhibited in the presence ofl-lysine. Treatment of cells with LPS and IFN-γ caused a reduction in intracellularl-arginine concentration which was accompanied by increases in the levels ofl-citrulline in both the cells and culture medium.

These findings indicate that activation of J774 cells with LPS produces an increase in bothl-arginine transport and nitrite synthesis. The elevated rate ofl-arginine transport in activated J774 cells may provide a mechanism for sustained substrate supply during enhanced utilization ofl-arginine for the generation of nitric oxide.

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Authors and Affiliations

  1. Vascular Biology Research Centre, Biomedical Sciences Division, King's College, Campden Hill Road, W8 7AH, London, UK

    A. R. Baydoun, R. G. Bogle, J. D. Pearson & G. E. Mann

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  1. A. R. Baydoun
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  2. R. G. Bogle
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  3. J. D. Pearson
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  4. G. E. Mann
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Baydoun, A.R., Bogle, R.G., Pearson, J.D. et al. Arginine uptake and metabolism in cultured murine macrophages. Agents and Actions 38 (Suppl 2), C127–C129 (1993). https://doi.org/10.1007/BF01991160

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  • DOI: https://doi.org/10.1007/BF01991160

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