Agents and Actions

, Volume 38, Supplement 2, pp C120–C121 | Cite as

15-Hydroxy-eicosatetraenoic acid has minor anti-inflammatory properties in colitis

  • A. P. M. van Dijk
  • D. M. McCafferty
  • J. H. P. Wilson
  • F. J. Zijlstra
General Inflammation


This study was performed to determine whether 15-HETE, which is the major metabolite of arachidonic acid (AA) in inflamed and normal human colonic tissue, has pro- or anti-inflammatory properties. To investigate these effects, 15-HETE (100 μg/kg/day) was administered rectally to mice with colitis induced by dextran sodium sulphate (DSS). Colons were removed and examined macroscopically and histologically and specimens were incubated with [14C]-AA and stimulated with A23187. Exogenous eicosanoids were separated by HPLC; the endogenously formed mediators were measured by radioimmunoassay. DSS produced a marked diffuse inflammatory response in the colon, associated with a raised inflammation score (mean 7.6 vs. <0.5) and a significant increase in endogenously formed metabolites PGE2, LTB4 and 12-HETE. 15-HETE treatment resulted in a slight decrease in inflammation score (6.4 vs. 7.6) and a slight, but not significant, decrease in endogenous LTB4.


Sulphate Inflammatory Response Arachidonic Acid Dextran PGE2 
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Copyright information

© Birkhäuser Verlag 1993

Authors and Affiliations

  • A. P. M. van Dijk
    • 1
  • D. M. McCafferty
    • 1
  • J. H. P. Wilson
    • 2
  • F. J. Zijlstra
    • 1
  1. 1.Dept. of PharmacologyErasmus UniversityRotterdamThe Netherlands
  2. 2.Dept. of Internal Medicine IIErasmus UniversityRotterdamThe Netherlands

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