15-Hydroxy-eicosatetraenoic acid has minor anti-inflammatory properties in colitis
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This study was performed to determine whether 15-HETE, which is the major metabolite of arachidonic acid (AA) in inflamed and normal human colonic tissue, has pro- or anti-inflammatory properties. To investigate these effects, 15-HETE (100 μg/kg/day) was administered rectally to mice with colitis induced by dextran sodium sulphate (DSS). Colons were removed and examined macroscopically and histologically and specimens were incubated with [14C]-AA and stimulated with A23187. Exogenous eicosanoids were separated by HPLC; the endogenously formed mediators were measured by radioimmunoassay. DSS produced a marked diffuse inflammatory response in the colon, associated with a raised inflammation score (mean 7.6 vs. <0.5) and a significant increase in endogenously formed metabolites PGE2, LTB4 and 12-HETE. 15-HETE treatment resulted in a slight decrease in inflammation score (6.4 vs. 7.6) and a slight, but not significant, decrease in endogenous LTB4.
KeywordsSulphate Inflammatory Response Arachidonic Acid Dextran PGE2
- F. J. Zijlstra, A. P. M. van Dijk, J. H. P. Wilson, I. C. van Riemsdijk-Overbeeke, J. E. Vincent and R. J. Th. Ouwendijk,15-HETE is the main eicosanoid formed by human colonic mucosa. Agents and Actions35, C53-C59 (1992).Google Scholar