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Agents and Actions

, Volume 38, Supplement 2, pp C120–C121 | Cite as

15-Hydroxy-eicosatetraenoic acid has minor anti-inflammatory properties in colitis

  • A. P. M. van Dijk
  • D. M. McCafferty
  • J. H. P. Wilson
  • F. J. Zijlstra
General Inflammation

Abstract

This study was performed to determine whether 15-HETE, which is the major metabolite of arachidonic acid (AA) in inflamed and normal human colonic tissue, has pro- or anti-inflammatory properties. To investigate these effects, 15-HETE (100 μg/kg/day) was administered rectally to mice with colitis induced by dextran sodium sulphate (DSS). Colons were removed and examined macroscopically and histologically and specimens were incubated with [14C]-AA and stimulated with A23187. Exogenous eicosanoids were separated by HPLC; the endogenously formed mediators were measured by radioimmunoassay. DSS produced a marked diffuse inflammatory response in the colon, associated with a raised inflammation score (mean 7.6 vs. <0.5) and a significant increase in endogenously formed metabolites PGE2, LTB4 and 12-HETE. 15-HETE treatment resulted in a slight decrease in inflammation score (6.4 vs. 7.6) and a slight, but not significant, decrease in endogenous LTB4.

Keywords

Sulphate Inflammatory Response Arachidonic Acid Dextran PGE2 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Birkhäuser Verlag 1993

Authors and Affiliations

  • A. P. M. van Dijk
    • 1
  • D. M. McCafferty
    • 1
  • J. H. P. Wilson
    • 2
  • F. J. Zijlstra
    • 1
  1. 1.Dept. of PharmacologyErasmus UniversityRotterdamThe Netherlands
  2. 2.Dept. of Internal Medicine IIErasmus UniversityRotterdamThe Netherlands

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