Abstract
We have studied the effect of platelet-activating factor (PAF) on the physico-chemical organization of human lymphocyte plasma membranes by measuring the steady-state fluorescence anisotropy and the fluorescence decay of 1-(4-trimethylammoniumphenyl)-6-phenyl-1,3,5-hexatriene (TMA-DPH) incorporated in lymphocyte plasma membranes. PAF induced a time-limited and significant increase of the lipid order in the exterior part of the membrane and a decrease in membrane heterogeneity. These changes were blocked in the presence of PAF antagonist L-659,989. The results indicate that the observed changes in the physico-chemical properties of the lymphocyte plasma membranes may be attributed to a PAF-receptor interaction.
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Kantar, A., Giorgi, P.L. & Fiorini, R. Effect of PAF on human lymphocyte membranes: A fluorescence study. Agents and Actions 38 (Suppl 2), C115–C117 (1993). https://doi.org/10.1007/BF01991155
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DOI: https://doi.org/10.1007/BF01991155