Abstract
A new model of the isolated perfused lung from different strains of mice was developed. Lungs from Swiss, Balb/C and CBA mice actively sensitised to ovalbumin were challenged intratracheally (i.t.) by antigen on day 14. In Swiss mice instillation of ovalbumin led to the release of leukotriene (LT) C4 significantly above basal values. Conversely, lungs from Balb/C and CBA mice were unresponsive to ovalbumin in terms of production of LTC4. All strains failed to release histamine when challenged with antigen. Intratracheal instillation of platelet-activating factor (PAF), to lungs from non-sensitised animals, induced the release of comparable amounts of LTC4, irrespective of the strain. In contrast, i.t. administration of fMLP to lungs from Swiss mice elicited release of significantly higher amounts of LTC4 as compared to Balb/C and CBA mice. In separate experiments, ovalbumin was injected into the paws and anaphylactic oedema was evaluated. Balb/C and CBA required 1 μg to show an oedema formation, whereas the dose of ovalbumin for Swiss mice to develop a similar response was at least 30-fold lower. In conclusion, antigen provocation induced release of LTC4 from lungs from Swiss mice but not from Balb/C or CBA. This difference may be accounted for by strain-dependent factors, such as antibody production and requires further investigation.
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References
R. D. R. Camp, A. A. Coutts, M. W. Greaves, A. B. Kay and M. J. Walport,Responses in human skin to intradermal injections of leukotrienes C 4,D 4,B 4. Br. J. Pharmacol.80, 497–502 (1983).
W. Leng, C. G. Kuo, R. Qureshi and B. A. Jakschik,Role of leukotrienes in vascular changes in the rat mesentery and skin in anaphylaxis. J. Immunol.140, 2361–2368 (1988).
J. M. Drazen, K. F. Austen, R. A. Lewis, D. A. Clark, G. Goto, A. Marfat and E. J. Corey,Comparative airway and vascular activities of leukotrienes C-1 and D in vivo and in vitro, Proc. Natl. Acad. Sci. USA77, 4354–4358 (1980).
S. E. Dahlen, J. Bjork, P. Hedqvist, K. E. Arfors, S. Hammarstrom, J. A. Lindgren and B. Samuelsson,Leukotrienes promote plasma leakage and leukocyte adhesion in postcapillary venules: In vivo effects with relevance to the acute inflammatory response. Proc. Natl. Acad. Sci. USA78, 3887–3891 (1981).
P. M. O'Byrne,Leukotrienes, airways hyperresponsiveness and asthma. InBiology of the Leukotrienes (Eds. R. Levi and R. D. Krell) pp. 282–290, Annals of the New York Academy of Sciences, New York 1988.
P. S. Creticos, S. P. Peters, N. F. Adkinson, R. M. Naclerio, E. C. Hayes, P. S. Norman and L. M. Lichtenstein,Peptido leukotriene release after antigen challenge in patients sensitive to ragweed. New Engl. J. Med.310, 1626–1630 (1984).
J. M. Mencia-Huerta, E. Razin, E. W. Ringel, E. J. Corey, D. Hoover, K. F. Austen and R. A. Lewis,Immunologic and ionophore-induced generation of leukotriene B4 from mouse bone marrow-derived mast cells. J. Immunol.130, 1885–1890 (1983).
U. Aehringhaus, R. H. Wolbling, W. Konig, C. Patrono, B. M. Peskar and B. A. Peskar,Release of leukotriene C 4 from human polymorphonuclear leukocytes as determined by radioimmunoassay. FEBS Letts.146, 111–114 (1982).
B. Lebel,A high-sampling-rate automated continuous-flow fluorimetric technique for the analysis of nanogram levels of histamine in biological samples. Anal. Biochem.133, 16–29 (1983).
S. H. Ferreira,A new method for measuring variations of rat paw volume. J. Pharm. Pharmacol.31, 648 (1979).
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Zuany-Amorim, C., Pretolani, M., Leduc, D. et al. Lung responsiveness to antigen in sensitised mice of different strains. Agents and Actions 38 (Suppl 2), C105–C107 (1993). https://doi.org/10.1007/BF01991152
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DOI: https://doi.org/10.1007/BF01991152