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Role of phagocytic synovial lining cells in experimental arthritis

  • General Inflammation
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Abstract

We investigated thein vivo role of phagocytic synovial lining cells (SLC) in the onset of experimental arthritis by depleting phagocytic SLC prior to arthritis induction. Phagocytic SLC were depleted by a single intra-articular injection of liposomes encapsulating the drug dichloromethylene diphosphonate (CL2MDP). Seven days after injection optimal depletion was observed and this time point was chosen for induction of arthritis in SLC depleted joints. Joint swelling was highly reduced after elicitation with either zymosan, immune complexes or antigen, as compared to observations in normal non-depleted joints. In addition cellular infiltration was markedly reduced. Further study in the immune complex mediated arthritis revealed that reduced cell influx in SLC depleted knee joints was correlated to lowered chemotactic activity and IL-1 levels as measured in washouts of joint tissues. This indicates that IL-1 driven chemotactic factors might be involved. Furthermore reduced cell influx was also correlated to significantly diminished loss of35S-prelabeled PG from the cartilage. Out data indicate that SLC are directly involved in the onset of joint inflammation.

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Authors and Affiliations

  1. Department of Rheumatology, University Hospital St. Radboud, Geert Grooteplein Zuid 8, 6500 HB, Nijmegen

    P. L. E. M. van Lent, A. E. M. van den Hoek, L. van den Bersselaar & W. B. van den Berg

  2. Department of Histology, Free University, Amsterdam

    N. van Rooijen

Authors
  1. P. L. E. M. van Lent
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  2. A. E. M. van den Hoek
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  3. L. van den Bersselaar
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  4. N. van Rooijen
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  5. W. B. van den Berg
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van Lent, P.L.E.M., van den Hoek, A.E.M., van den Bersselaar, L. et al. Role of phagocytic synovial lining cells in experimental arthritis. Agents and Actions 38 (Suppl 2), C92–C94 (1993). https://doi.org/10.1007/BF01991148

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  • DOI: https://doi.org/10.1007/BF01991148

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