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Inflammatory mediators and activity of human peritoneal macrophages

  • General Inflammation
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Abstract

Human peritoneal macrophages (hp-Mφ) are a source of inflammatory mediators. After stimulationin vitro for 24 h with LPS there was a significant increase in cytokine production (IL-1, IL-6 and TNFα), but not in the production of eicosanoids from endogenous arachidonate. Leukotrienes are the predominant eicosanoids formed after stimulation with calcium ionophore for 15 min, while prostaglandin formation is insignificant. The fluorescence intensity of TPA-stimulated and DHR123 loaded hp-Mφ (a measure of the respiratory burst) increases significantly in a short period of time. Hp-Mφ will be useful as a model for testing the effects of anti-inflammatory drugs on eicosanoid and cytokine production and respiratory burst activityin vitro.

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Authors and Affiliations

  1. Department of Pharmacology, Erasmus University, P.O. Box 1738, 3000 DR, Rotterdam

    W. M. Pruimboom, A. P. M. van Dijk, C. J. A. M. Tak, F. J. Zijlstra & I. L. Bonta

  2. Department of Internal Medicine II, Academic Hospital Dijkzigt, Rotterdam, The Netherlands

    J. H. P. Wilson

Authors
  1. W. M. Pruimboom
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  2. A. P. M. van Dijk
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  3. C. J. A. M. Tak
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  4. F. J. Zijlstra
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  5. I. L. Bonta
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  6. J. H. P. Wilson
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Pruimboom, W.M., van Dijk, A.P.M., Tak, C.J.A.M. et al. Inflammatory mediators and activity of human peritoneal macrophages. Agents and Actions 38 (Suppl 2), C86–C88 (1993). https://doi.org/10.1007/BF01991146

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  • DOI: https://doi.org/10.1007/BF01991146

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