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, Volume 38, Supplement 2, pp C83–C85 | Cite as

Does TNF-α directly increase endothelial cell monolayer permeability?

  • A. Burke-Gaffney
  • A. K. Keenan
General Inflammation

Abstract

The effects of dexamethasone (DEX) and ϖ methyl ester (l-NAME) on the tumour necrosis factor-α (TNF-α)-induced increase in permeability of human umbilical vein endothelial cell (HUVEC) monolayer to [125I] labelled bovine serum albumin (BSA) were examined. Preincubation of HUVEC monolayers with DEX (1μM, 2 h) completely abolished the effect of TNF-α (5 ng/ml, 18 h). Administration of DEX 2 h after TNF-α also reduced the effect of TNF-α, whilel-NAME (5 ng/ml, 1 mM, 18 h) had no significant effect.

The observed inhibition of the TNF-α-induced permeability increase on preincubation with DEX would suggest a role for nitric oxide (NO) in mediating the permeability response. However, this is not confirmed by the experiments withl-NAME. The inhibition caused by DEX administered after TNF-α would suggest alternative mechanisms by which DEX may be acting in addition to inhibition of NO synthase induction.

Keywords

Methyl Ester Nitric Oxide Bovine Serum Albumin Tumour Necrosis 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

References

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Copyright information

© Birkhäuser Verlag 1993

Authors and Affiliations

  • A. Burke-Gaffney
    • 1
    • 2
  • A. K. Keenan
    • 2
  1. 1.Children's Research CentreOur Lady's Hospital for Sick ChildrenDublin 12
  2. 2.Department for PharmacologyUniversity College DublinDublin 4Ireland

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