Skip to main content
SpringerLink
Log in

Inhibition of the acute-phase response in a human hepatoma cell line

  • Future Trends in the Therapy of Inflammatory Disease
  • Published:
Agents and Actions Aims and scope Submit manuscript

Abstract

The HuH-7 human hepatoma cell line was stimulated by IL-1 and IL-6 to increase the synthesis of acutephase proteins, e.g. serum amyloid A (SAA),α1 antichymotrypsin (ACT), α1-protease inhibitor, α1 acid-glycoprotein and haptoglobin, with the exception of the pentraxins (serum amyloid P and C-reactive protein). Haptoglobin and ACT were stimulated by IL-1 which has not been observed in some other hepatoma cell lines. The concentration of IL-1 required for stimulation of SAA was higher than that required for haptoglobin stimulation. IL-1 receptor antagonist was capable of inhibiting these responses and acted at a lower concentration to inhibit SAA than required to inhibit ACT or haptoglobin induction. Transforming growth factor β (TGFβ) was also able to inhibit the response to IL-1 but had no effect on acute-phase protein responses to IL-6.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. P. C. Heinrich, J. V. Castell and T. Andus,Interleukin-6 and the acute phase response. Biochem. J.265, 621–636 (1989).

    Google Scholar 

  2. J. G. Raynes, S. Eagling and K. P. W. J. McAdam,Acute phase protein synthesis in human hepatoma cells: Differential regulation of SAA and haptoglobin by IL-1 and IL-6. Clin. Exp. Immunol.84, 488–492 (1991).

    PubMed  Google Scholar 

  3. S. Bevan and J.G. Raynes,IL-1 receptor antagonist regulation of acute phase protein synthesis in human hepatoma cells. J. Immunol.147, 2547–2578 (1991).

    PubMed  Google Scholar 

  4. C. M. Dubois, F. W. Ruscetti, E.W. Palaszinynski, L. A. Falk, J. J. Oppenheim and J. R. Keller,Transforming growth factor β is a potent inhibitor of IL-1 receptor expression; proposed mechanism of inhibition of IL-1 action. J. Exp. Med.172, 737–744 (1990).

    PubMed  Google Scholar 

  5. M. Turner, D. Chantry, P. Katsikis, A. Berger, F. Brennan and M. Feldmann,Induction of the interleukin-1 receptor antagonist protein by transforming growth factor-β. Eur. J. Immunol.21, 1635–1639 (1991).

    PubMed  Google Scholar 

  6. G. Morrone, R. Cortese and V. Sorrentino,Post-transcriptional control of negative acute phase genes by TGFβ. EMBO J.8, 3767–3771 (1989).

    PubMed  Google Scholar 

  7. A. Makiewicz, M.K. Ganapathi, D. Schultz, A. Brabanec, J. Weinstein, M.F. Kelley and I. Kushner,TGFβ1 regulates production of acute phase proteins. Proc. Natl. Acad. Sci. USA87, 1491–1495 (1990).

    PubMed  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Molecular Immunology, Clinical Sciences, London School of Hygiene and Tropical Medicine, WCIE 7HT, London, UK

    J. G. Raynes & S. Bevan

Authors
  1. J. G. Raynes
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. S. Bevan
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Raynes, J.G., Bevan, S. Inhibition of the acute-phase response in a human hepatoma cell line. Agents and Actions 38 (Suppl 2), C66–C68 (1993). https://doi.org/10.1007/BF01991140

Download citation

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF01991140

Keywords

Search

Navigation