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Dextran sulphate inhibits neutrophil emigration and neutrophil-dependent plasma leakage in rabbit skin

  • Cell Adhesion Molecules in Inflammation
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Abstract

Rolling of neutrophils in postcapillary venules is believed to be an obligatory step in the adhesion of neutrophils to endothelial cells, preceding firm attachment and emigration. This rolling on the endothelial surface can be blocked by sulphated polysaccharides. Therefore, dextran sulphate or dextran T500 was injected intravenously (i.v., 25 mg/kg) and neutrophil infiltration and plasma leakage in skin were measured in response to intradermal (i.d.) injection of inflammatory mediators in the presence of calcitonin gene-related peptide (CGRP). Neutrophil accumulation and oedema formation induced by the neutrophil chemoattractants, f-Met-Leu-Phe (FMLP) or C5adesArg, were completely suppressed in the presence of dextran sulphate. Neutrophil emigration in response to interleukin-1 (IL-1) was not affected by dextran sulphate. These results suggest that neutrophil recruitment induced by FMLP and C5adesArg, but not by IL-1, is mediated via sulphated glycans, perhaps on endothelial cells.

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Author notes

  1. N. Van Osselaer & M. Rampart (Senior Research Associate)

    Present address: the National Fund for Scientific Research, Belgium

Authors and Affiliations

  1. Division of Pharmacology, Faculty of Medicine, University of Antwerp (UIA), B2610, Wilrijk, Belgium

    N. Van Osselaer, A. G. Herman (Research Assistant) & M. Rampart (Senior Research Associate)

Authors
  1. N. Van Osselaer
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  2. A. G. Herman
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  3. M. Rampart
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Van Osselaer, N., Herman, A.G. & Rampart, M. Dextran sulphate inhibits neutrophil emigration and neutrophil-dependent plasma leakage in rabbit skin. Agents and Actions 38 (Suppl 2), C51–C53 (1993). https://doi.org/10.1007/BF01991134

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  • DOI: https://doi.org/10.1007/BF01991134

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