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, Volume 38, Supplement 2, pp C47–C50 | Cite as

Observations of the time-course of the inflammatory response of rectal mucosa to gliadin challenge in gluten-sensitive subjects

  • A. Ensari
  • A. Ager
  • M. N. Marsh
  • S. Morgan
  • K. Moriarty
Cell Adhesion Molecules in Inflammation

Abstract

Expression of endothelial adhesion molecules is a key factor in localizing inflammatory processes. We have evaluated the time-course expression of E-selectin, ICAM-1, and VCAM-1 in rectal mucosae from coeliac patients (CD) with T-lymphocyte sensitization to wheat (gliadin) protein. Ten treated, eight untreated CD patients and five disease controls underwent a dynamic 4 h rectal gluten challenge. Seven treated CD patients had a 24 h challenge to evaluate the time-course changes. Coeliac tissue significantly increased VCAM-1 expression [p<0.001 (untreated and treated CD)] and E-selectin [p=0.01 (untreated CD only)]-expression compared to controls but not ICAM-1. VCAM-1 continued to increase up to 24 h, while E-selectin decreased after 4 h. These findings relate to an 8 h rise in CD3 (+) lymphocytes [p<0.05 (treated)] and a 4 h rise in neutrophils (p<0.005). This approach permits a novel study of the inflammatory response to a known immunogen in human patients.

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Copyright information

© Birkhäuser Verlag 1993

Authors and Affiliations

  • A. Ensari
    • 1
  • A. Ager
    • 2
  • M. N. Marsh
    • 1
  • S. Morgan
    • 1
  • K. Moriarty
    • 1
  1. 1.University Department of MedicineUniversity of Manchester School of Medicine, Hope HospitalSalfordUK
  2. 2.University Department of Cellular ImmunologyUniversity of Manchester School of Medicine, Hope HospitalSalfordUK

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