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Changes in neutrophil CD11b/CD18 andl-selectin expression and release of interleukin 8 and elastase in paediatric cardiopulmonary bypass

  • Cell Adhesion Molecules in Inflammation
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Abstract

Children undergoing cardiopulmonary bypass (CPB) surgery for congenital heart defects develop an acute post-operative capillary leak which may be due to endothelial injury inflicted by adherent neutrophils (PMN). Direct immunofluorescence and flow cytometry were used to measure CD11a/CD18, CD11b/CD18 andl-selectin (l-s) expression on circulating PMN in CPB circuits containing human blood and in children undergoing CPB.In vitro, a general rise in CD11b/CD18 expression over 2h contrasted with complete loss ofl-s in a small but progressively increasing proportion of PMN. Marked but inconsistent changes in CD11b/CD18 andl-s were observedin vivo, in conjunction with fluctuations in circulating PMN count. Circulating IL-8 was detected starting at rewarming from hypothermia and reperfusion of the heart and lungs with a simultaneous, closely correlated rise in both PMN count and circulating elastase. IL-1 and TNF were not detected. These studies demonstrate changes in the pathways of PMN-endothelial interaction during and after CPB.

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Authors and Affiliations

  1. Dept. of Paediatrics, The Sheffield Children's Hospital, The University of Sheffield, S10 2TH, Sheffield, UK

    Adam Finn

  2. The Hospital for Sick Children and Institute of Child Health, WC1N, London, UK

    Naomi Rebuck, Stephan Strobel & Martin Elliott

  3. Brompton Hospital, SW3, London, UK

    Neil Moat

  4. Dept. Paediatrics, St. Mary's Hospital, W2, London, UK

    Nigel Klein

Authors
  1. Adam Finn
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  2. Neil Moat
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  3. Naomi Rebuck
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  4. Nigel Klein
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  5. Stephan Strobel
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  6. Martin Elliott
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Finn, A., Moat, N., Rebuck, N. et al. Changes in neutrophil CD11b/CD18 andl-selectin expression and release of interleukin 8 and elastase in paediatric cardiopulmonary bypass. Agents and Actions 38 (Suppl 2), C44–C46 (1993). https://doi.org/10.1007/BF01991132

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  • DOI: https://doi.org/10.1007/BF01991132

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