Advertisement

Agents and Actions

, Volume 38, Supplement 2, pp C22–C24 | Cite as

Role of nitric oxide in the vasodilator but not exudative component of mustard oil-induced inflammation in rat skin

  • I. T. Lippe
  • A. Stabentheiner
  • P. Holzer
Neurogenic Pain and Inflammation

Abstract

The participation of nitric oxide (NO) in the neurogenic inflammatory reaction of the rat hindpaw skin to topical application of mustard oil was examined by the use ofNG-nitro-l-arginine methyl ester (l-NAME, 43 μmol kg−1 i.v.), an inhibitor of NO formation. Control rats received the same dose of the inactive enantiomerd-NAME. Vasodilatation was recorded by contactless infrared emission thermography, and plasma protein exudation was measured by the Evans Blue leakage technique and by measurement of the paw volume in anaesthetized rats.l-NAME reduced the cutaneous hyperaemia caused by topical administration of mustard oil by about 50% but did not change the exudative reaction to mustard oil. These findings indicate that NO plays a mediator role in the vasodilator component of neurogenic inflammation in the rat paw skin, whereas the increase in vascular permeability does not appear to depend on NO.

Keywords

Nitric Oxide Evans Blue Neurogenic Inflammation Infrared Emission Mediator Role 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

References

  1. [1]
    S. M. Louis, A. Jamieson, N. J. W. Russell and G. J. Dockray,The role of substance P and calcitonin gene-related peptide in neurogenic plasma extravasation and vasodilatation in the rat. Neuroscience32, 581–586 (1989).PubMedGoogle Scholar
  2. [2]
    G. Burnstock,Local mechanisms of blood flow control by perivascular nerves and endothelium, J. Hypertens.8, S95-S106 (1990).Google Scholar
  3. [3]
    V. Ralevic, Z. Khalil, G. J. Dusting and R. D. Helme,Nitric oxide and sensory nerves are involved in the vasodilator response to acetylcholine but not calcitonin gene-related peptide in rat skin microvasculature. Br. J. Pharmacol.106, 650–655 (1992).PubMedGoogle Scholar
  4. [4]
    S. R. Hughes, T. J. Williams and S. D. Brain,Evidence that endogenous nitric oxide modulates oedema formation induced by substance P. Eur. J. Pharmacol.191, 481–484 (1990).PubMedGoogle Scholar
  5. [5]
    A. Stabentheiner and S. Schmaranzer,Thermographic determination of body temperatures in honey bees and hornets: Calibration and applications. Thermology2, 563–572 (1987).Google Scholar
  6. [6]
    F. Lembeck and P. Holzer,Substance P as neurogenic mediator of antidromic vasodilation and neurogenic plasma extravasation. Naunyn-Schmiedeberg's Arch. Pharmacol.310, 175–183 (1979).Google Scholar
  7. [7]
    S. Moncada, R. M. J. Palmer and E. A. Higgs,Nitric oxide: Physiology, pathophysiology, and pharmacology. Pharmacol. Rev.43, 109–142 (1991).PubMedGoogle Scholar

Copyright information

© Birkhäuser Verlag 1993

Authors and Affiliations

  • I. T. Lippe
    • 1
  • A. Stabentheiner
    • 2
  • P. Holzer
    • 1
  1. 1.Department of PharmacologyUniversity of GrazGrazAustria
  2. 2.Department of ZoologyUniversity of GrazGrazAustria

Personalised recommendations