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, Volume 36, Supplement 1, pp C76–C78 | Cite as

Experimental colitis in mice: Effects of olsalazine on eicosanoid production in colonic tissue

  • F. J. Zijlstra
  • I. M. Garrelds
  • A. P. M. van Dijk
  • J. H. P. Wilson
Experimental Aspects of Gastrointestinal Inflammation New Approaches to Therapy of Gastrointestinal Inflammation General Inflammation Research Topics 3rd Meeting on Side Effects of Anti-Inflammatory Drugs and 13th European Workshop on Inflammation Verona, Italy Short Communication Gastrointestinal Inflammation

Abstract

In rodents colitis can be induced by adding 2% (w/v) carrageenan (CARR) for 4 weeks or 10% (w/v) dextran sulphate sodium (DSS) for 7 days to their drinking water. These models are suitable to test antiinflammatory drugs used in inflammatory bowel disease in man.

Mice were treated with olsalazine (400 mg/kg body wt) starting 7 days before the DSS or CARR administration. Colonic tissues were incubated with [1-14C]-arachidonic acid and stimulated with A23187 and, thereafter, the pattern of eicosanoids was determined by separation on HPLC.

DSS and CARR produced a marked diffuse inflammatory response in the colon and a subsequent 5-fold increase of all eicosanoids after DSS, whereas after CARR only a 2-fold increase of PGs was observed. Olsalazine treatment decreased all cyclooxygenase and lipoxygenase products to baseline levels.

Keywords

Drinking Water Inflammatory Bowel Disease Arachidonic Acid Dextran Bowel Disease 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

References

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Copyright information

© Birkhäuser Verlag 1992

Authors and Affiliations

  • F. J. Zijlstra
    • 1
  • I. M. Garrelds
    • 1
  • A. P. M. van Dijk
    • 1
  • J. H. P. Wilson
    • 2
  1. 1.Department of PharmacologyErasmus UniversityRotterdamThe Netherlands
  2. 2.Department of Internal Medicine IIErasmus UniversityRotterdamThe Netherlands

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