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, Volume 36, Supplement 1, pp C76–C78 | Cite as

Experimental colitis in mice: Effects of olsalazine on eicosanoid production in colonic tissue

  • F. J. Zijlstra
  • I. M. Garrelds
  • A. P. M. van Dijk
  • J. H. P. Wilson
Experimental Aspects of Gastrointestinal Inflammation New Approaches to Therapy of Gastrointestinal Inflammation General Inflammation Research Topics 3rd Meeting on Side Effects of Anti-Inflammatory Drugs and 13th European Workshop on Inflammation Verona, Italy Short Communication Gastrointestinal Inflammation


In rodents colitis can be induced by adding 2% (w/v) carrageenan (CARR) for 4 weeks or 10% (w/v) dextran sulphate sodium (DSS) for 7 days to their drinking water. These models are suitable to test antiinflammatory drugs used in inflammatory bowel disease in man.

Mice were treated with olsalazine (400 mg/kg body wt) starting 7 days before the DSS or CARR administration. Colonic tissues were incubated with [1-14C]-arachidonic acid and stimulated with A23187 and, thereafter, the pattern of eicosanoids was determined by separation on HPLC.

DSS and CARR produced a marked diffuse inflammatory response in the colon and a subsequent 5-fold increase of all eicosanoids after DSS, whereas after CARR only a 2-fold increase of PGs was observed. Olsalazine treatment decreased all cyclooxygenase and lipoxygenase products to baseline levels.


Drinking Water Inflammatory Bowel Disease Arachidonic Acid Dextran Bowel Disease 
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  1. [1]
    P. Sharon and W. F. Stenson,Enhanced synthesis of leukotriene B 4 by colonic mucosa in inflammatory bowel disease. Gastroenterology86, 453–60 (1984).PubMedGoogle Scholar
  2. [2]
    J. Vilaseca, A. Salas, F. Guarner, R. Rodriguez and J.-R. Malagelada,Participation of thromboxane and other eicosanoid synthesis in the course of experimental inflammatory colitis. Gastroenterology98, 269–77 (1990).PubMedGoogle Scholar
  3. [3]
    I. Okayasu, S. Hatakeyama, M. Yamada, T. Ohkusa, Y. Inagaki and R. Nakaya,A novel method in the induction of reliable experimental acute and chronic ulcerative colitis in mice. Gastroenterology98, 694–702 (1990).Google Scholar
  4. [4]
    J. M. Langman, R. Rowland and B. Vernon-Roberts,Carrageenan colitis in the guinea pig: Pathological changes and the importance of ascorbic acid deficiency in disease induction. Aust. J. Exp. Biol. Med. Sci.63, 545–53 (1985).PubMedGoogle Scholar
  5. [5]
    A. C. Van der Ham, W. J. Kort, A. M. Bijma, F. J. Zijlstra, M. A. Vermeer and J. Jeekel,Eicosanoid profile of healing colon anastomosis and peritoneal macrophages in the rat. Gut31, 807–11 (1990).PubMedGoogle Scholar

Copyright information

© Birkhäuser Verlag 1992

Authors and Affiliations

  • F. J. Zijlstra
    • 1
  • I. M. Garrelds
    • 1
  • A. P. M. van Dijk
    • 1
  • J. H. P. Wilson
    • 2
  1. 1.Department of PharmacologyErasmus UniversityRotterdamThe Netherlands
  2. 2.Department of Internal Medicine IIErasmus UniversityRotterdamThe Netherlands

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