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Organophosphate-detoxicating enzymes inE. coli. Gelfiltration and isoelectric focusing of DFPase, paraoxonase and unspecific phosphohydrolases

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Zusammenfassung

Die Entgiftung von Organophosphaten durchE. coli-Phosphophydrolasen wurde untersucht und eine DFPase (E.C. 3.8.2.1) und eine Paraoxonase (E.C. 3.1.1.2) nachgewiesen. Die Trennung der Phosphohydrolasen erfolgte durch Gelfiltration und die isoelektrischen Punkte wurden in freier, präparativer Fokussierung bestimmt. Ausserdem wurden Substrat- und pH-Abhängigkeit untersucht.

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Authors and Affiliations

  1. Physiologisch-chemisches Institut der Universität, Humboldtallee 7, D-34, Göttingen, Germany

    R. Zech & K. D. Wigand

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  1. R. Zech
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  2. K. D. Wigand
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Zech, R., Wigand, K.D. Organophosphate-detoxicating enzymes inE. coli. Gelfiltration and isoelectric focusing of DFPase, paraoxonase and unspecific phosphohydrolases. Experientia 31, 157–158 (1975). https://doi.org/10.1007/BF01990678

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