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Purification of porcine granulosa cells by continuous Percoll gradient

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Summary

Granulosa cells purified on a continuous Percoll gradient have considerably increased responsiveness to FSH in formation of cAMP, secretion of progesterone, and125I [hCG] binding to cells incubated for 4 days in culture.

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References

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Authors and Affiliations

  1. Institute of Experimental Endocrinology, Centrum of Physiological Sciences, Slovak Academy of Sciences, CS-809 36, Bratislava, Czechoslovakia

    J. Kolena, A. Kiss & C. P. Channing

  2. Department of Physiology, University of Maryland School of Medicine, 21201, Baltimore, Maryland, USA

    J. Kolena, A. Kiss & C. P. Channing

Authors
  1. J. Kolena
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  2. A. Kiss
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  3. C. P. Channing
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Additional information

This investigation received financial support from the World Health Organization.

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Kolena, J., Kiss, A. & Channing, C.P. Purification of porcine granulosa cells by continuous Percoll gradient. Experientia 39, 908–909 (1983). https://doi.org/10.1007/BF01990432

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  • DOI: https://doi.org/10.1007/BF01990432

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