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Transmembrane potential of J774.2 mouse macrophage cells measured by microelectrode and ion distribution methods

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Summary

The transmembrane potential (Em) of J774.2 macrophage cells measured by microelectrodes was −24.1±0.7 mV (mean±SEM). Em measured by lipophilic ion distribution was −35±2 mV or −40±2 mV, using a cation or anion, respectively. By any method, colchicine reduced Em by ∼3 mV.

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Author notes

  1. D. J. McCaig

    Present address: Department of Pharmacology, University of Otago, P. O. Box 913, Dunedin, New Zealand

Authors and Affiliations

  1. Department of Physiology, University of Connecticut Health Center, 06032, Farmington, Connecticut, USA

    D. J. McCaig & R. D. Berlin

Authors
  1. D. J. McCaig
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  2. R. D. Berlin
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Additional information

Acknowledgments. Supported by grants CA15544, HL23192 and CA18564 from NIH. Our especial appreciation to Dr J.M. Oliver whose generosity made possible these experiments.

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McCaig, D.J., Berlin, R.D. Transmembrane potential of J774.2 mouse macrophage cells measured by microelectrode and ion distribution methods. Experientia 39, 906–907 (1983). https://doi.org/10.1007/BF01990431

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  • DOI: https://doi.org/10.1007/BF01990431

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