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Cytogenetic toxicity of D2O in human lymphocyte cultures. Increased sensitivity in Fanconil's anemia

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Summary

Chromosomal aberrations were scored in lymphocyte cultures from healthy individuals, patients with Bloom syndrome, and patients with Fanconi's anemia, after 4–5 h exposure to culture medium containing 90% heavy water (D2O). D2O treatment resulted in occasional pulverization of metaphases, and increased frequencies of chromosomal breakage. Patients with Fanconi's anemia were particularly sensitive to the chromosome breaking effect of D2O.

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References

  1. Gross, P.R., and Spindel, W., Ann. N.Y. Acad. Sci.84 (1960) 745.

    Article  CAS  PubMed  Google Scholar 

  2. Murphy, J., Desaive, C., Giaretti, W., Kendall, F., and Nicolini, C., J. Cell Sci.25 (1977) 87.

    Article  CAS  PubMed  Google Scholar 

  3. Moorhead, P.S., Nowell, P.C., Mellman, W.J., Battips, D.M., and Hungerford, D.A., Exp. Cell Res.20 (1960) 613.

    Article  CAS  PubMed  Google Scholar 

  4. International System for Human Cytogenetic Nomenclature 1978 Cytogenet. Cell Genet.21 (1978) 309.

    Article  Google Scholar 

  5. German, J., Bloom, D., and Passarge, E., Clin. Genet.12 (1977) 162.

    Article  CAS  PubMed  Google Scholar 

  6. Hustinx, T.W.J., Ter Haar, B. G. A., Scheres, J.M.J.C., Rutten, F.J., Weemaes, C.M.R., Hoppe, R.L.E., and Jansen, A.H., Clin. Genet.12 (1977) 85.

    Article  CAS  PubMed  Google Scholar 

  7. Sasaki, M. S., and Tonomura, A., Cancer Res.33 (1973) 1829.

    CAS  PubMed  Google Scholar 

  8. Schroeder, T.M., Cytogenet. Cell Genet.33 (1982) 119.

    Article  CAS  Google Scholar 

  9. Williams, D.M., Scott, C.D., and Beck, T.M., Blood47 (1976) 687.

    Article  CAS  PubMed  Google Scholar 

  10. Matsui, S., Yoshida, H., Weinfeld, H., and Sanders, A.A., J. Cell Biol.54 (1972) 120.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  11. Thomson, J.F., Ann. N.Y. Acad. Sci.84 (1960) 736.

    Article  CAS  PubMed  Google Scholar 

  12. Merkel, P. B., and Kearns, D. R., J. Am. chem. Soc.94 (1972) 1029.

    Article  CAS  Google Scholar 

  13. Bielski, B. H. J., and Saito E.., J. Phys. Chem.75 (1971) 2263.

    Article  CAS  Google Scholar 

  14. Fridovich, I., Science201 (1978) 875.

    Article  CAS  PubMed  Google Scholar 

  15. Joenje, H., Arwert, F., Eriksson, A. W., de Koning, H., and Oostra, A. B., Nature290 (1981) 142.

    Article  CAS  PubMed  Google Scholar 

  16. Emerit, I. and Cerutti, P.A., Proc. natl Acad. Sci. USA78 (1981) 1868.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

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Authors and Affiliations

  1. Institute of Human Genetics, Free University, P.O. Box 7161, NL-1007 MC, Amsterdam, The Netherlands

    H. Joenje, A. B. Oostra & A. H. Wanamarta

Authors
  1. H. Joenje
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  2. A. B. Oostra
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  3. A. H. Wanamarta
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Additional information

We gratefully acknowledge the cooreration of Dr C.M.R. Weemaes (Nijmegen) for the cases of Bloom syndrome, and Dr J.J. de Koning (Leiden) and Dr K.W. Roozendaal (Amsterdam) for the Fanconi's anemia cases. We thank Fré Arwert and Aggie Nieuwint for helpful comments on the manuscript and Piet Kostense for statistical advice.

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Joenje, H., Oostra, A.B. & Wanamarta, A.H. Cytogenetic toxicity of D2O in human lymphocyte cultures. Increased sensitivity in Fanconil's anemia. Experientia 39, 782–784 (1983). https://doi.org/10.1007/BF01990325

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