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Chromatographic separation of coelomic fluid fromHolothuria polii (Echinodermata) and partial characterization of the fractions reacting with erythrocytes

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Summary

Coelomic fluid preparations fromHolothuria polii were passed through a Bio-gel A5m column. The 3 separated protein peaks possess hemagglutinating or hemolytic activity against rabbit erythrocytes. Electrophoretic and immunochemical methods showed that 2 identical protein subunits characterized hemagglutinins of different size. Hemolysin differs from hemagglutinin in molecular weight and organization of subunits.

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Authors and Affiliations

  1. Zoological Institute, Palermo University, Via Archirafi 18, I-90123, Palermo, Italy

    C. Canicatti & N. Parrinello

Authors
  1. C. Canicatti
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  2. N. Parrinello
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Additional information

We would like to thank Prof. B. Salvato, Institute of Animal Biology, University of Padova, for his critical reading of the manuscript. Reprint requests to N.P.

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Canicatti, C., Parrinello, N. Chromatographic separation of coelomic fluid fromHolothuria polii (Echinodermata) and partial characterization of the fractions reacting with erythrocytes. Experientia 39, 764–766 (1983). https://doi.org/10.1007/BF01990315

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  • DOI: https://doi.org/10.1007/BF01990315

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