Skip to main content
SpringerLink
Log in

Intracellular topography of immunoreactive gastrin demonstrated using electron immunocytochemistry

  • Full Papers
  • Published:
Experientia Aims and scope Submit manuscript

Summary

Gastrin (G)-producing cells from the mammalian gastric antrum have been investigated using computer-assisted morphometry and a novel double colloidal gold-labeled-immunoglobulin electron immunocytochemical procedure. Correlation analysis of human antral G-cells indicates (p<0.001) that a single population of granules exists with small (160 nm) electron-dense and large (240 nm) electron-lucent forms representing the extremes. Non-crossreacting region-specific antisera have been used to visualize G-17 and G-34 (progastrin) to the small electron-dense granules and G-17 to the other intermediate forms. From the results we propose a topographic segregation of immunoreactive gastrins within 2 apparently distinct granule subclasses and suggest that this may represent the pathway of granule maturation.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Bryant, M. G., and Adrian, T. E., Gastrin, in: Radioimmunassay of gut regulatory peptides, pp. 51–59. Eds S. R. Bloom and R. G. Long. W. B. Saunders, London 1982.

    Google Scholar 

  2. De Mey, J., Moeremans, M., Geuens, G., Nuydens, R., and De Brabander, M., High resolution light and electron microscopic localization of tubulin with the IGS (immunogold staining) method. Cell Biol. Int. Rep.5 (1981) 889–899.

    Article  PubMed  Google Scholar 

  3. Dockray, G. J., Gregory, R. A., Tracy, H. J., and Zhu, W.-Y., Postsecretory processing of heptadecapeptide gastrin: Conversion to C-terminal immunoreactive fragments in the circulation of the dog. Gastroenterology83 (1982) 224–232.

    Article  CAS  PubMed  Google Scholar 

  4. Forssmann, W. G., Feuerle, G. E., Yanaihara, N., Helmstaedter, V., Glotzer, J., Buchler, M., Greenberg, J., Tischbirek, K., Lehy, T., Ito, S., and Lichtenberger, L. M., Ultrastructure and immunocytochemistry of gastrin cells (G cells). Biomed. Res.1 (1980) suppl. 54, 54–66.

    CAS  Google Scholar 

  5. Forssmann, W. G., and Orci, L., Ultrastructure and secretory cycle of the gastrin-producing cell. Z. Zellforsch. mikrosk. Anat.101 (1969) 419–432.

    Article  CAS  PubMed  Google Scholar 

  6. Forssmann, W. G., Orci, L., Pictet, R., and Rouiller, C., Zur Ultrastruktur der endokrinen Zellen im Epithel des Magendarmtraktes der Ratte. Acta anat.68 (1967) 605–606.

    Google Scholar 

  7. Geoghegan, W. D., and Ackerman, G. A., Adsorption of horseradish peroxidase, ovomucoid and anti-immunoglobulin to colloidal gold for the indirect detection of concanavalin A, wheat germ agglutinin and goat anti-human immunoglobulin G on cell surfaces at the electron microscopic level: a new method, theory and application. J. Histochem. Cytochem.25 (1977) 1187–1200.

    Article  CAS  PubMed  Google Scholar 

  8. Gregory, R. A., and Tracy, H. J., The constitution and properties of two gastrins extracted from hog antral mucosa. Gut5 (1964) 103–114.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  9. Grube, D., and Aebert, H., Immunocytochemical investigations of the gastroenteropancreatic endocrine cells using semithin and thin serial sections, in: Cellular basis of chemical messengers in the digestive system. pp. 83–95. Eds M. Grossman, M. Brazier and J. Lechago. Academic Press, New York 1981.

    Chapter  Google Scholar 

  10. Håkanson, R., Alumets, J., Rehfeld, J. F., Ekelund, M., and Sundler, F., The life cycle of the gastrin granule. Cell Tissue Res.222 (1982) 479–491.

    Article  PubMed  Google Scholar 

  11. Harris, A., Lackie, P. M., Tapia, F. J., Varndell, I. M., and Polak, J. M., Secretory granule profiles: a quantitative approach to electron microscopy. J. Path. (1983) in press.

  12. Iwanaga, T., Kusumoto, Y., Fujita, T., Yanaihara, C., Mochizuki, T., and Yanaihara, N., Immunocytochemical localization of the different gastrin forms in the pyloric antrum. Biomed. Res.1 (1980) 316–320.

    Article  CAS  Google Scholar 

  13. Larsson, L.-I., Immunocytochemical characterization of ACTH-like immunoreactivity in cerebral nerves and in endocrine cells of the pituitary and gastrointestinal tract by using region-specific antisera. J. Histochem. Cytochem.28 (1980) 133–141.

    Article  CAS  PubMed  Google Scholar 

  14. Larsson, L.-I., and Rehfeld, J. F., Characterization of antral gastrin cells with region-specific antisera. J. Histochem. Cytochem.25 (1977) 1317–1321.

    Article  CAS  PubMed  Google Scholar 

  15. Lund, P. K., Goodman, R. H., Dee, P. C., and Habener, J. F., Pancreatic preproglucagon cDNA contains two glucagon-related coding sequences arranged in tandem. Proc. natl Acad. Sci. USA79 (1982) 345–349.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  16. Moody, A. J., Holst, J. J., Thim, L., and Lindkaer Jensen, S., Relationship of glicentin to proglucagon and glucagon in the porcine pancreas. Nature289 (1981) 514–516.

    Article  CAS  PubMed  Google Scholar 

  17. Mortenson, N. J. McC., The anatomy of the gastrin cell. Ann. R. Coll. Surg.62 (1980) 462–469.

    Google Scholar 

  18. Mortenson, N. J. McC., Morris, J. F., and Owens, C., Gastrin and the ultrastructure of G cells in the fasting rat. Gut20 (1979) 41–50.

    Article  Google Scholar 

  19. Munger, B. L., Caramia, F. and Lacy, P. E. The ultrastructural basis for the identification of cell types in the pancreatic islets. II. Rabbit, dog and opossum. Z. Zellforsch. mikrosk. Anat.67 (1965) 776–798.

    Article  CAS  PubMed  Google Scholar 

  20. Osaka, M., Sasagawa, T., and Fujita, T., Emiocytotic granule release in the human antral endocrine cells, in: Gastro-Entero-Pancreatic Endocrine System, pp. 59–63. Ed. T. Fujita. Thieme, Stuttgart 1974.

    Google Scholar 

  21. Polak, J. M., and Buchan, A. M. J., Heterogeneity of the D1 cell, in: Cellular basis of chemical messengers in the digestive system, pp. 121–131. Eds. M. Grossman, M. Brazier and J. Lechago. Academic Press, New York 1981.

    Chapter  Google Scholar 

  22. Probert, L., De Mey, J., and Polak, J. M., Distinct subpopulations of enteric p-type neurones contain substance P and vasoactive intestinal polypeptide. Nature294 (1981) 470–471.

    Article  CAS  PubMed  Google Scholar 

  23. Ravazzola, M., and Orci, L., Glucagon and glicentin immunoreactivity are topologically segregated in thea granule of the human pancreatic A cell. Nature284 (1980) 66–68.

    Article  CAS  PubMed  Google Scholar 

  24. Rehfeld, J. F., and Uvnas-Wallensten, K., Gastrins in cat and dog: Evidence for a biosynthetic relationship between the large molecular forms of gastrin and heptadecapeptide gastrin. J. Physiol.283 (1978) 379–396.

    Article  CAS  Google Scholar 

  25. Romano, E. L., and Romano, M., Staphylococcal protein A bound to colloidal gold: A useful reagent to label antigen-antibody sites in electron microscopy. Immunochemistry14 (1977) 711–715.

    Article  CAS  Google Scholar 

  26. Romano, E. L., Stolinski, C., and Hughes-Jones, N. C., An anti-globulin reagent labelled with colloidal gold for use in electron microscopy. Immunochemistry11 (1974) 521–522.

    Article  CAS  PubMed  Google Scholar 

  27. Roth, J., Bendayan, M., and Orci, L., Ultrastructural localization of intracellular antigens by the use of protein A-gold complex. J. Histochem. Cytochem.26 (1978) 1074–1081.

    Article  CAS  PubMed  Google Scholar 

  28. shields, D., Warren, T. G., Roth, S. E., and Brenner, M. J., Cell-free synthesis and processing of multiple precursors to glucagon. Nature289 (1981) 511–514.

    Article  CAS  PubMed  Google Scholar 

  29. Solcia, E., Vassallo, G., Capella, C., and Sampietro, R., Endocrine cells in the antro-pyloric mucosa of the stomach. Z. Zellforsch. mikrosk. Anat.81 (1967) 474–486.

    Article  CAS  PubMed  Google Scholar 

  30. Tapia, F. J., Varndell, I. M., De Mey, J., Heding, L., Yanaihara, N., Bloom, S. R., and Polak, J. M., Demonstration of immunoreactive sites of insulin biosynthesis using immunogold procedures. Gut23 (1982) A883.

    Google Scholar 

  31. Tapia, F. J., Varndell, I. M., Probert, L., De Mey, J., and Polak, J. M., Double immunogold staining method for the simultaneous ultrastructural localisation of regulatory peptides. J. Histochem. Cytochem.31 (1983) in press.

  32. Track, N. S., Creutzfeldt, C., Arnold, R., and Creutzfeldt, W., The antral gastrin-producing G-cell: Biochemical and ultrastructural responses to feeding. Cell Tissue Res.194 (1978) 131–139.

    Article  CAS  PubMed  Google Scholar 

  33. Vaillant, C., Dockray, G., and Hopkins, C. R., Cellular origins of different forms of gastrin: The specific immunocytochemical localisation of related peptides. J. Histochem. Cytochem.27 (1979) 932–935.

    Article  CAS  PubMed  Google Scholar 

  34. Varndell, I. M., Tapia, F. J., Probert, L., Buchan, A. M. J., Gu, J., De Mey, J., Bloom, S. R., and Polak, J. M., Immunogold staining procedure for the localisation of regulatory peptides. Peptides3 (1982) 259–272.

    Article  CAS  PubMed  Google Scholar 

Download references

Author information

Author notes

  1. N. Yanaihara

    Present address: National Institute for Physiological Science, Okasaki, Japan

  2. J. De Mey

    Present address: Laboratory of Oncology, Department of Life Sciences, Janssen Pharmaceutica, B-2340, Beerse, Belgium

Authors and Affiliations

  1. National Institute for Physiological Science, Okasaki, Japan

    I. M. Varndell

  2. Department of Histochemistry and Medicine, Royal Postgraduate Medical School, Hammersmith Hospital, Du Cane Road, W12 OHS, London, England

    A. Harris, F. J. Tapia, N. Yanaihara, J. De Mey, S. R. Bloom & J. M. Polak

Authors
  1. I. M. Varndell
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. A. Harris
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. F. J. Tapia
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. N. Yanaihara
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. J. De Mey
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. S. R. Bloom
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. J. M. Polak
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Varndell, I.M., Harris, A., Tapia, F.J. et al. Intracellular topography of immunoreactive gastrin demonstrated using electron immunocytochemistry. Experientia 39, 713–717 (1983). https://doi.org/10.1007/BF01990288

Download citation

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF01990288

Keywords

Search

Navigation