Summary
Three methods are evaluated for holding capsules of plastic-embedded tissue for rotary microtomy. Use of a V-block is rapid but deforms the capsule. Gluing the capsule to a supporting block is useful for reorientation of the capsule but otherwise time consuming. A metal adapter is easy to use, does not deform the tissue, and is the preferred method for routine microtomy.
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Supported in part by the U.S. Veterans Administration Hospital, Hines, Illinois.
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Cole, M.B. Holding plastic-embedded specimens for sectioning in a rotary microtome. Experientia 32, 674–675 (1976). https://doi.org/10.1007/BF01990229
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DOI: https://doi.org/10.1007/BF01990229