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Development of hybrid plants from ovules ofNicotiana tabacum pollinated in vitro with pollen grains ofNicotiana knightiana

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Summary

Hybrid embryos and endosperm were developed in ovules ofNicotiana tabacum after pollination in vitro with pollen grains ofNicotiana knightiana. Embryos produced calluses which after transferring to fresh medium differentiated into shoots which later formed plants. In 8 plants among 14 fully-developed plants growing in pots the chromosome numbers were 2n=36; the remaining 6 plants were aneuploids. All plants were male and female sterile.

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Authors and Affiliations

  1. Institute of Plant Genetics, Polish Academy of Science, Strzeszyńska 30/36, 60-479, Poznań, Poland

    A. Ślusarkiewicz-Jarzina & M. Zenkteler

  2. Department of General Botany, Institute of Biology, Adam Mickiewicz University, PL-61-713, Poznań, Poland

    M. Zenkteler

Authors
  1. A. Ślusarkiewicz-Jarzina
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  2. M. Zenkteler
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Ślusarkiewicz-Jarzina, A., Zenkteler, M. Development of hybrid plants from ovules ofNicotiana tabacum pollinated in vitro with pollen grains ofNicotiana knightiana. Experientia 39, 1399–1400 (1983). https://doi.org/10.1007/BF01990125

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  • DOI: https://doi.org/10.1007/BF01990125

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