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Inhibition of erythrocyte ATPase activity by aclacinomycin and reverse effects of ascorbate on ATPase activity

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Summary

We studied the effect of aclacinomycin on human erythrocyte membrane enzymes. Aclacinomycin inhibited ATPase, including Na−K-dependent ATPase, ouabain insensitive ATPase and Ca-ATPase. However acetylcholinesterase was not inhibited by aclacinomycin. The ATPase activities were not inhibited by aclacinomycin if ascorbate was added to the incubation mixture. However other reducing agents, α-tocopherol, superoxide dismutase and catalase had no effect on ATPase activity. Ascorbate may protect membrane proteins and lipids from peroxidate damage.

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Authors and Affiliations

  1. Department of Medicine, Kanazawa University School of Medicine, 920, Kanazawa, Japan

    T. Kitao & K. Hattori

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  1. T. Kitao
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  2. K. Hattori
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Kitao, T., Hattori, K. Inhibition of erythrocyte ATPase activity by aclacinomycin and reverse effects of ascorbate on ATPase activity. Experientia 39, 1362–1364 (1983). https://doi.org/10.1007/BF01990105

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  • DOI: https://doi.org/10.1007/BF01990105

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