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Quantitative assay for testing susceptibility of HIV isolates to zidovudine and sCD4 (178)-PE40

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Abstract

A quantitative coculture assay is described for determining susceptibility of HIV-1 isolates to zidovudine and sCD4 (178)-PE40 (a 60 kDa hybrid protein consisting of the gp120 binding region of CD4 linked to the translocation and ADP-ribosylation regions ofPseudomonas aeruginosa exotoxin). The assay was relatively simple to perform and gave highly reproducible results often within three to five days. IC50 values of zidovudine for HIV-1 strains isolated from two AIDS patients and an asymptomatic seropositive individual were in the range 0.001–0.002 µmol. Isolates obtained after six months of zidovudine treatment had zidovudine IC50 values of 0.01–0.5 µmol. All isolates were equally sensitive to sCD4 (178)-PE40 (IC50 1–5 µg/ml). HIV-1 activation in the chronically infected cell line U-1 was inhibited by sCD4 (178)-PE40 but not by zidovudine.

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Authors and Affiliations

  1. Laboratory for Medical Microbiology, University Hospital, Room G04.515, PO Box 85500, 3508 GA, Utrecht, The Netherlands

    J. Verhoef

  2. Department of Medicine, Hennepin County Medical Center, Minneapolis, Minnesota, USA

    G. Gekker & P. K. Peterson

  3. Department of Laboratory Medicine and Pathology, University of Minnesota, Minneapolis, Minnesota, USA

    A. Erice & H. H. Balfour

Authors
  1. J. Verhoef
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  2. G. Gekker
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  3. A. Erice
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  4. P. K. Peterson
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  5. H. H. Balfour
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Verhoef, J., Gekker, G., Erice, A. et al. Quantitative assay for testing susceptibility of HIV isolates to zidovudine and sCD4 (178)-PE40. Eur. J. Clin. Microbiol. Infect. Dis. 11, 715–721 (1992). https://doi.org/10.1007/BF01989976

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