Agents and Actions

, Volume 18, Issue 1–2, pp 266–268 | Cite as

Histamine and mucosal mast cells in gluten enteropathy

  • Urban Wingren
  • Claes Hallert
  • Klas Norrby
  • Lennart Enerbäck
Histamine in Pathological States

Abstract

Coeliac disease is a malabsorptive disorder caused by intolerance to gluten and is characterized by a remodelling of the intestinal mucosa including villus atrophy, crypt hyperplasia and net increase of mucosal volume. Changes of the number of mucosal mast cells (MMCs) in coeliac mucosa has recently been reported, suggesting that the mast cell activity could have a pathogenetic role in gluten enteropathy. MMCs located solely in the lamina propria are the main repository for small-gut mucosal histamine.

A consecutive prospective study was designed to study the histamine content, MMC numbers, and the relative volume of lamina propria in intestinal biopsies from adult patients suffering from unexplained diarrhea and/or malnutrition. Histamine was measured by a HPLC-method, the number of MMC was counted after long toluidine-blue staining, and the relative volumes of lamina propiria and epithelium were estimated morphometrically. The findings were correlated to the histopathological appearance of the mucosa. As compared to controls the histamine content increased by 80% and MMC numbers by about 60% in the coeliac mucosa. There was also a correlation between MMC numbers and histamine content for both normal and coeliac mucosae (r=0.81). The morphometric estimation of the relative volumes of epithelium and lamina propria revealed that the lamina-propria compartment was increased by approximately 40% in coeliac mucosa.

Taking the changes in compartmental volumes of the remodelled coeliac mucosa into account, our results suggest that the histamine content and MMC population were significantly increased. MMC and MMC-associated histamine may therefore be involved in the pathogenesis of gluten enteropathy.

Keywords

Histamine Lamina Propria Coeliac Disease Mast Cell Activity Histamine Content 

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. [1]
    N. Wright, M. Alison,The biology of epithelial cell populations. Vol. 2. pp. 769–773. Oxford University Press, New York, (1984).Google Scholar
  2. [2]
    P. Kumar, D.P. O'Donoghee, M. Lancaster-Smith,Cellular changes in the jejunal mucosa following the reintroduction of gluten in treated coeliac disease. In: The mast cell, its role in health and disease. Ed J. Pepys, A.M. Edwards, Pitman Medical Publishing Co Ltd, pp. 647–650 (1979).Google Scholar
  3. [3]
    S. Strobel, A. Busuttil, A. Fergusson,Human intestinal mucosal mast cells: expanded population in untreated coeliac disease, Gut24, 222–227 (1983).PubMedGoogle Scholar
  4. [4]
    M.N. Marsh, J. Hinde,Inflammatory component of celiac sprue mucosa. I. Mast cells, basophils, and eosinophils, Gastroenterology89, 92–101 (1985).PubMedGoogle Scholar
  5. [5]
    L. Dolberg, M. Gevitz, S. Freier,Gastrointestinal mast cells in health, and in coeliac disease and other conditions, Arch Dis Child55, 702–705 (1980).PubMedGoogle Scholar
  6. [6]
    I. Kosnai, P. Kuitunen, E. Savilahti, P. Sipponen,Mast cells in small intestine in cow's milk protein intolerance and in coeliac disease, Pediatr Res.15, 1201 (1981).Google Scholar
  7. [7]
    J.O.D. Alexander,The small intestine in dermatitis herpetiformis, In: Major problems in dermatology4, 236–280 (1975).Google Scholar
  8. [8]
    C. Hallert, R. Gotthard, K. Norrby, A. Walan,On the prevalence of adult coeliac disease in Sweden, Scand J Gastroenterol16, 257–261 (1981).PubMedGoogle Scholar
  9. [9]
    U. Wingren, L. Enerbäck,Mucosal mast cells of the rat intestine: a re-evaluation of fixation and staining properties, with special reference to protein blocking and solubility of the granular glycosaminoglycan, Histochem J15, 571–582 (1983).CrossRefPubMedGoogle Scholar
  10. [10]
    S. Allenmark, S. Bergström, L. Enerbäck,A selective post-column o-phthaladehyde-derivatization system for the determination of histamine in biological material by HPLC, Anal Biochem144, 98–103, (1985).CrossRefPubMedGoogle Scholar
  11. [11]
    O.H. Lowry, N.J. Rosebrough, A.L. Farr, R.J. Randall,Protein measurement with the Folin phenol reagent, J Biol Chem193, 265–275 (1951).PubMedGoogle Scholar

Copyright information

© Birkhäuser Verlag 1986

Authors and Affiliations

  • Urban Wingren
    • 1
  • Claes Hallert
    • 2
  • Klas Norrby
    • 1
  • Lennart Enerbäck
    • 1
  1. 1.Department of PathologyUniversity of Göteborg, Sahlgren HospitalGöteborgSweden
  2. 2.Department of Internal MedicineCentral HospitalVäxjöSweden

Personalised recommendations