IgE-mediated activation of human heartin vitro
- 16 Downloads
We used human cardiac tissue from the right atrial appendages of patients undergoing corrective heart surgery to study content andde novo synthesis of mediators in the human heart. Human heart tissue contained 1.7±0.1 μg/g wet weight of histamine (mean±S.E.M.) and spontaneously produced 6-keto-PGF1α (38.4 ng/g wet weight/min), PGF2α (1.9 ng/g wet weight/min), PGE (1.7 ng/g wet weight/min) and thromboxane B2 (T×B2) (1.7 ng/g wet weight/min). Spontaneous release of PGD2, leukotriene C4 and histamine was negligible. Rabbit anti-human IgE (1–10 μg/ml) dose-dependently induced the release of histmaine (5 to 15% of the total histamine content) and of PGD2 (5 to 100 ng/g of wet tissue). The effect of anti-IgE was dose-related and reached a maximum after 30–45 min of incubation. A significant linear correlation (rs=0.90; p<0.001) was found betweende novo synthesis of PGD2 and the secretion of histamine induced by anti-IgE challenge of human heart. These results support the concept that PGI2 is the main, but not the sole, product of arachidonic acid metabolism synthesized by human heartin vitro. Additionally, anti-IgE challenge of human heartin vitro induces the release of histamine and PGD2. The local concentrations of these mediators appear high enough to play some role in the modulation of several cardiac functionsin vivo.
KeywordsHistamine Arachidonic Acid Thromboxane Human Heart PGI2
Unable to display preview. Download preview PDF.
- G. Marone, M. Triggiani, R. Cirillo, E. de Lutio di Castelguidone, M. Chiariello andM. Condorelli,The role of chemical mediators in ischemic cardiac syndromes. In: Atherosclerosis and Cardiovascular Diseases. Aetiopathogenesis, Epidemiology, Clinical Evaluation, Prevention and Therapy, pp. 315–333 (Eds. S. Lenzi and G.C. Descovich), MTP Medical Press, Lancaster, 1984.Google Scholar
- G. Marone,The role of basophils and mast cells in the pathogenesis of pulmonary diseases, Int. Arch. Allergy Appl. Immun.76, 70–82, (1985).Google Scholar
- R.M. Robertson, D. Robertson, G.A. FitzGerald andJ.A. Oates,The role of prostaglandins in ischemic cardiac syndromes. In Advances in Clinical Immunology. The Role of Chemical Mediators in Pulmonary and Cardiac Diseases, pp. 173–189 (Eds. M. Condorelli, G. Marone and L.M. Lichtenstein), OIC Medical Press, Florence, 1984.Google Scholar
- W. Lorenz, A. Doenicke, R. Meyer, H.J. Reimann, J. Kusche, H. Barth, H. Geesing, M. Hutzel andB. Weissembacher,Histamine release in man by propanidid and thiopentone: pharmacological effects and clinical consequences, Br. J. Anaest.44, 355–368, (1972).Google Scholar
- M. Condorelli, G. Marone andL.M. Lichtenstein,Advances in Clinical Immunology. The Role of Chemical Mediators in Pulmonary and Cardiac Diseases, O.I.C. Medical Press, Florence, 1984.Google Scholar
- G. Marone, A. Kagey-Sobotka andL.M. Lichtenstein,IgE-mediated histamine release from human basophils: differences between antigen E- and anti-IgE-induced secretion, Int. Arch. Allergy Appl. Immun.65, 339–348 (1981).Google Scholar
- R. Levi, D.A.A. Owen andJ. Trzeciakowski,Action of histamine on the heart and vasculature. In: Pharmacology of Histamine Receptors, pp. 236–297 (Eds. C.R. Ganellin and M.E. Parsons). Wright-P.S.G., 1982.Google Scholar
- C. Vigorito, P. Russo, G.B. Picotti, M. Chiariello, S. Poto andG. Marone,Cardiovascular effects of histamine infusion in man, J. Cardiov. Pharmacol.5, 531–537 (1983).Google Scholar