Some properties of mast cells obtained by human bronchoalveolar lavage
- 21 Downloads
The properties of human pulmonary mast cells obtained by enzymic dispersion of whole lung and by bronchoalveolar lavage (BAL) have been compared with those of the basophil leucocyte. The latter cell types responded with release of histamine to challenge with anti-human IgE but the dispersed cells reacted only after passive sensitisation with serum from an atopic donor. Disodium cromoglycate inhibited the release of histamine from both types of pulmonary mast cell although the characteristics of the inhibition were different in the two cases. The drug was ineffective against the basophil. Increased numbers of mast cells were recovered by lavage of asthmatic subjects and these cells responded to immunological challenge with an enhanced release of histamine. The possible clinical significance of these findings in human bronchial asthma is discussed.
KeywordsAsthma Mast Cell Histamine Disodium Bronchoalveolar Lavage
Unable to display preview. Download preview PDF.
- J. Morley,Mediators and cyclic nucleotides in asthma. InAsthma, pp. 285–300 (EdsT.J.H. Clark andS. Godfrey). Chapman and Hall, London 1983.Google Scholar
- L.M. Lichtenstein andA.G. Osler,Studies on the mechanisms of hypersensitivity phenomena. IX. Histamine release from human leukocytes by ragweed pollen antigen, J. exp. Med.120, 507–530 (1964).Google Scholar
- G. Atkinson, M. Ennis andF.L. Pearce,The effect of alkaline earth cations on the release of histamine from rat peritoneal mast cells treated with compound 48/80 and peptide 401, Br. J. Pharmac.65, 395–402, (1979).Google Scholar