Advertisement

Agents and Actions

, Volume 18, Issue 1–2, pp 49–52 | Cite as

Distribution of actin filaments in rat mast cells and its role in histamine release

  • K. Tasaka
  • M. Akagi
  • K. Miyoshi
Histamine Release

Abstract

The distribution of action filaments was confirmed by immunofluorescence microscopy and immuno-electron microscopy in nonsecreting and secreting rat peritoneal mast cells. In a resting cell, immunofluorescence of the actin showed up as a net-like formation surrounding each granule. After stimulation with compound 48/80 or A-23187, the distribution of the actin filaments became very irregular and disordered, disappearing completely in some areas within the cell and expanding in conjunction with the swelling of the granules. Similar changes are seen in a passively sensitized mast cell exposed to antigen. When cells were pretreated with cytochalasin B or colchicine, the distribution of actin filaments was not greatly affected by stimulation. The results clearly show that actin filaments exist in mast cells.

Keywords

Mast Cell Histamine Colchicine Actin Filament Histamine Release 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. [1]
    T.S.C. Orr, D.E. Hall andA.C. Allison,Role of contraction microfilaments in the release of histamine from mast cells, Nature236, 350–351 (1972).PubMedGoogle Scholar
  2. [2]
    P. Röhlich,Membrane-associated actin filaments in the cortical cytoplasm of the rat mast cell, Exp. Cell. Res.93, 293–298 (1975).CrossRefPubMedGoogle Scholar
  3. [3]
    E.H. Nielsen andH. Jahn,Cytoskeletal studies on Lowicryl K4M embedded and Affi-Gel 731 attached rat peritoneal mast cells, Virchows Arch.45, 313–323 (1984).Google Scholar
  4. [4]
    A. Nemeth andP. Röhlich,Rapid separation of rat peritoneal mast cells with Percoll, Eur. J. Cell Biol.20, 272–275 (1980).PubMedGoogle Scholar
  5. [5]
    P.A. Shore, Fluorometric assay histamine. InMethods in Enzymology, Vol. 17/b, pp. 842–845 (EdsH. Tabor andC.W. Tabor). Academic Press, New York 1971.Google Scholar
  6. [6]
    M. Akagi andK. Tasaka,Lateral diffusion of membrane particles at the early stage in IgE-mediated mast cell degranulation, J. Electron Microsc.33, 371–374 (1984).Google Scholar
  7. [7]
    D. Lagunoff andE.Y. Chi,Effect of colchicine on rat mast cells, J. Cell. Biol.71, 182–195 (1976).CrossRefPubMedGoogle Scholar
  8. [8]
    W. Wolf, A. Deboben, F.A. Bautz, H. Faulstich andTh. Wieland,Fluorescent phallatoxin, a tool for the visualization of cellular actin, Proc. Natl. Acad. Sci.76, 4498–4502 (1979).PubMedGoogle Scholar

Copyright information

© Birkhäuser Verlag 1986

Authors and Affiliations

  • K. Tasaka
    • 1
  • M. Akagi
    • 1
  • K. Miyoshi
    • 1
  1. 1.Department of Pharmacology, Faculty of Pharmaceutical SciencesOkayama UniversityOkayamaJapan

Personalised recommendations