Skip to main content
SpringerLink
Log in

Polarized light scattering from biological systems: A technique for cell differentiation

  • Published:
Journal of Biological Physics Aims and scope Submit manuscript

Abstract

Measurements of polarized light scattered from spores, bacterial cells, and red and white blood cells in various conditions yield optical data that can differentiate between species, strains, surface characteristics, killing methods, antibody binding, red blood cell types, and two types of human white blood cells. The light scattering instrument used, which operates in the context of the Mueller matrix/Stokes vector description of the scattering process, is described. One particular matrix element, S34, is especially sensitive to small changes and cellular differences.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  • Beverly, P. C. 1976. In B AND T CELLS IN IMMUNE RECOGNITION (F. Loor and G. E. Boleants, eds.). John Wiley & Sons, New York, p. 35.

    Google Scholar 

  • Bickel, W. S. 1979. Appl. Optics18, 1707.

    Google Scholar 

  • Biehl, R. B. L. M.S. Thesis, University of Arizona, 1979.

  • Bickel, W. S.; Davidson, J. F.; Huffman, D. R.; Kilkson, R. 1976. Proc. Nat. Acad. Sci. USA,73, 486.

    Google Scholar 

  • Bickel, W. S.; Stafford, M. E. 1980. In LIGHT SCATTERING BY IRREGULARLY SHAPED PARTICLES (D. W. Schuerman, ed.). Plenum Publishing Corp., New York, pp. 299–305.

    Google Scholar 

  • Hunt, A. J.; Huffman, D. R. 1973. Rev. Sci. Instrum.44, 1753.

    Article  Google Scholar 

  • Mie, G. 1908. Ann. Phys. (Leipzig)25, 377.

    Google Scholar 

  • Mueller, H. 1948. J. Opt. Soc. Am.38, 661.

    Google Scholar 

  • Lord Rayleigh 1911. Proc. Roy. Soc. (London)A 85, 25.

    Google Scholar 

  • Stokes, G. G. 1852. Trans. Camb. Phil. Soc.9, 399.

    Google Scholar 

  • Tanooka, H.; Terano, H.; Otsuka, H. 1971. Biochim. Biophys. Acta228, 26.

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Physics, University of Arizona, Tucson, Arizona

    W. S. Bickel & M. E. Stafford

Authors
  1. W. S. Bickel
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. M. E. Stafford
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Bickel, W.S., Stafford, M.E. Polarized light scattering from biological systems: A technique for cell differentiation. J Biol Phys 9, 53–66 (1981). https://doi.org/10.1007/BF01987283

Download citation

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF01987283

Keywords

Search

Navigation