Attempts to isolate a kallikrein inhibitor from rat salivary gland
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Besides the oxytocic principle (kallikrein) it was possible to separate from the rat submandibular gland extract an inhibitor presenting a specific antagonism against the oxytocic action of the submandibular gland kallikrein.
A protein fraction collected from sephadex G-100 column showed maximal oxytocic activity, with an OD at about 500 and an inhibitory effect with an OD at about 1700, indicated by absorption at 280 mμ.
Since the inhibitor appears in fractions following those holding the kallikrein activity, it is suggested that it has a lower molecular size than kallikrein.
The concentration of the inhibitor in the gland increases parallel with the development of animal.
KeywordsSalivary Gland Molecular Size Protein Fraction Submandibular Gland Gland Extract
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