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, Volume 41, Issue 1–2, pp 84–85 | Cite as

Model forin vitro investigation of bone mineralization

  • Gábor G. Ecsedi
Papers Presented at the Joint Meeting of the European Inflammation Society and Hungarian Biochemical Society Balatonaliga, Hungary, 19–21 May, 1993


Anin vitro model was developed for the investigation of bone cell mineralization processes. Out-grown cells of explanted human nasal bone fragments were selected into three lineages and one of them, the osteoblast-enriched line, was partially characterized. A large number of cells of this line stained readily for alkaline phosphatase. They produced prostanoids, PGl2, PGF and PGE2, and osteocalcin in the presence of 1,25(OH)2D3. Cells synthesized collagen and mineralized the developed extracellular matrix. This latter line seems to be appropriate as a model system to find effective compounds which increase bone calcification.

Key words

Human nasal bone cell culture Osteoblastenriched cell line In vitro mineralization 


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Copyright information

© Birkhäuser Verlag 1994

Authors and Affiliations

  • Gábor G. Ecsedi
    • 1
  1. 1.Cell Biology LaboratoriesResearch Centre of CHINOIN Pharmaceutical and Chemical Works Ltd.BudapestHungary

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