Model forin vitro investigation of bone mineralization
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Anin vitro model was developed for the investigation of bone cell mineralization processes. Out-grown cells of explanted human nasal bone fragments were selected into three lineages and one of them, the osteoblast-enriched line, was partially characterized. A large number of cells of this line stained readily for alkaline phosphatase. They produced prostanoids, PGl2, PGF2α and PGE2, and osteocalcin in the presence of 1,25(OH)2D3. Cells synthesized collagen and mineralized the developed extracellular matrix. This latter line seems to be appropriate as a model system to find effective compounds which increase bone calcification.
Key wordsHuman nasal bone cell culture Osteoblastenriched cell line In vitro mineralization
- L. Feuer, P. Barát, I. Strauss and E. Kékes,Experimental studies on the cardiological effects of ipriflavone on the isolated rabbit heart, and rat and dog. Arzneimittel Forsch. Drug Res.31, 953–958 (1981).Google Scholar
- N. Udagawa, T. Sasaki, T. Akatsu, N. Takahashi, S. Tanaka, T. Tamura, H. Tanaka and T. Suda,Lack of bone resorption in osteosclerotic (oc/oc) mice is due to a defect in osteoclast progenitors rather than the local microenvironment provided by osteoblastic cells. Biochem. Biophys. Res. Commun.184, 67–72 (1992).CrossRefPubMedGoogle Scholar