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The role of the cysteinyl and one of the tryptophyl residues in the neurotoxic action of suberitine

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Summary

The selective photooxidation of the single cysteinyl residue and one of the 3 tryptophyl residues of suberitine has been performed by irradiation using crystal violet and proflavine respectively as photosensitizers. Crystal violet and the protein form a 1∶1 complex with a consequent partial inhibition of the neurotoxic activity. The latter is completely abolished by specific photooxidation of cysteine which is probably involved in the active site of the protein. The modification of the tryptophyl residue induces a large loss of the activity as a consequence of a photoinduced extensive denaturation of suberitine.

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Authors and Affiliations

  1. Laboratory of Biochemistry, Stazione Zoologica, Villa Comunale, I-80121, Napoli, (Italy)

    L. Cariello, B. Salvato & G. Jori

  2. C.N.R. Center for the Physiology and Biochemistry of Hemocyanins and other Metalloproteins, Istituto di Biologia Animale, I-35100, Padova, (Italy)

    L. Cariello, B. Salvato & G. Jori

Authors
  1. L. Cariello
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  2. B. Salvato
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  3. G. Jori
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Cariello, L., Salvato, B. & Jori, G. The role of the cysteinyl and one of the tryptophyl residues in the neurotoxic action of suberitine. Experientia 37, 801–803 (1981). https://doi.org/10.1007/BF01985648

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  • DOI: https://doi.org/10.1007/BF01985648

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