, Volume 50, Issue 2, pp 159–163 | Cite as

Physical characteristics of the cerebral big prothoracicotropic hormone fromManduca sexta

  • D. P. Muehleisen
  • E. J. Katahira
  • R. S. Gray
  • W. E. Bollenbacher
Research Articles


The prothoracicotropic hormones (PTTHs) are cerebral peptides that control insect postembryonic development by stimulating the prothoracic glands to synthesize ecdysteroids. InManduca sexta, the tobacco hornworm, two classes of PTTH are distinguished by their Mr, small (ca. 7 kDa) and big PTTH (ca. 25–30 kDa). Little is known about the physical nature of the PTTHs and this study takes a first step towards defining characteristics of theManduca big PTTH. The neurohormone has a Stokes radius of 2.59 nm and a sedimentation coefficient of 2.76 S. Based on these data, an Mr of 29,443.7 and anf/f0 of 1.27 were calculated. Combined, the physical data revealManduca big PTTH is an asymmetrical acidic homodimeric peptide with intra- and intermolecular disulfide bonds.

Key words

Prothoracicotropic hormone Manduca sexta anti-PTTH monoclonal antibody sedimentation coefficient frictional ratio molecular weight 


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Copyright information

© Birkhäuser Verlag 1994

Authors and Affiliations

  • D. P. Muehleisen
    • 1
  • E. J. Katahira
    • 1
  • R. S. Gray
    • 1
  • W. E. Bollenbacher
    • 1
  1. 1.Department of BiologyUniversity of North Carolina at Chapel HillChapel HillUSA

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