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Gap junctional intercellular communication of cultured rat liver parenchymal cells is stabilized by epithelial cells and their isolated plasma membranes

Experientia volume 50, pages 124–126 (1994)Cite this article

Abstract

The gap junctional intercellular communication (GJIC) determined by measuring dye coupling with Lucifer yellow, decreased within 3 d from 66% to 28% in monocultures of rat liver parenchymal cells. Coculturing of the parenchymal cells with a nonparenchymal epithelial cell line from rat liver resulted in increased and stabilized intercellular communication (83% after 3 d). The presence of isolated plasma membrane vesicles of the nonparenchymal epithelial cells also stabilized the intercellular communication between the liver parenchymal cells (70% after 3 d). When liver parenchymal cells were cocultured with a rat liver fibroblast cell line the gap junctional communication between the parenchymal cells was not stabilized (43% after 3 d), and isolated plasma membrane vesicles of the fibroblast were also unable to support the GJIC in parenchymal cells (35% after 3 d). It is concluded that plasma membrane constituents of the nonparenchymal epithelial cells were responsible for the stabilization of the GJIC between parenchymal cells. A heterotypic gap junctional communication between parenchymal and nonparenchymal cells was not observed.

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Author notes

  1. D. Utesch

    Present address: Abt. Toxikologie, Frankfurter Straße 250, D64271, Darmstadt, Germany

Affiliations

  1. Institut für Toxikologie der Universität Mainz, Obere Zahlbacher Straße 67, D-55131, Mainz, Germany

    B. Diener, N. Beer, H. Dürk, M. Traiser, D. Utesch, R. J. Wieser & F. Oesch

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  1. B. Diener
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  2. N. Beer
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  3. H. Dürk
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  4. M. Traiser
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  5. D. Utesch
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  6. R. J. Wieser
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  7. F. Oesch
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Diener, B., Beer, N., Dürk, H. et al. Gap junctional intercellular communication of cultured rat liver parenchymal cells is stabilized by epithelial cells and their isolated plasma membranes. Experientia 50, 124–126 (1994). https://doi.org/10.1007/BF01984948

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  • DOI: https://doi.org/10.1007/BF01984948

Key words

  • Rat hepatocyte
  • gap junctional intercellular communication
  • coculture
  • plasma membrane
  • stabilization