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Evaluation of a commercial enzyme immunomembrane filter assay for detection of respiratory syncytial virus in clinical specimens

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Abstract

A commercial enzyme immunomembrane filter assay (EIFA) for respiratory syncytial virus (RSV) was compared prospectively with isolation in cell culture and an enzyme immunoassay. A total of 595 respiratory specimens, mostly from pediatric patients, were examined. The EIFA was 70.96 % sensitive and 72.40 % specific in comparison with cell culture. Results for 40 specimens (6.72 %) were uninterpretable, mainly due to filtration difficulties. Twenty-one (25 %) of 84 specimens whose results were initially considered false-positive were subsequently confirmed positive after a blocking test with bovine anti-RSV serum. On the basis of the total number of confirmed positive results, the sensitivity and the specificity of the test were 87.90 % and 75.77 %, respectively.

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Authors and Affiliations

  1. Department of Virology, Brugmann University Hospital, 4 Place Van Gehuchten, 1020, Brussels, Belgium

    D. Van Beers, M. De Foor, L. Di Cesare & C. Vandenvelde

Authors
  1. D. Van Beers
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  2. M. De Foor
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  3. L. Di Cesare
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  4. C. Vandenvelde
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Van Beers, D., De Foor, M., Di Cesare, L. et al. Evaluation of a commercial enzyme immunomembrane filter assay for detection of respiratory syncytial virus in clinical specimens. Eur. J. Clin. Microbiol. Infect. Dis. 10, 1073–1076 (1991). https://doi.org/10.1007/BF01984934

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  • DOI: https://doi.org/10.1007/BF01984934

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