Abstract
From 1983 to 1989, 520Escherichia coli blood culture pathogens were isolated from two hospitals in Turku, Finland. Ampicillin resistance (MIC≥16 µg/ml) of these isolates increased from 33 % in 1983 to 66 % in 1987, but decreased to 38–49 % in 1988–1989. Occurrence of TEM-1 beta-lactamase producing isolates increased only slightly from 14 % in 1983 to 25 % in 1989 among allEscherichia coli strains studied. Strains with ampicillin MIC values of 16 µg/ml and 32 µg/ml were mostly responsible for the increase in resistance. Among these isolates, TEM-1 or any other of the well known plasmid-mediated beta-lactamases were not found by hybridization or isoelectric focusing.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Medeiros AA, Jacoby GA Beta-lactamase mediated resistance. In: Queener SF, Webber JA, Queener SW (ed): Beta-lactam antibiotics for clinical use. Marcel Dekker, New York, 1986, p. 49–84.
Philippon A, Labia R, Jacoby GA Extended-spectrum beta-lactamases. Antimicrobial Agents and Chemotherapy 1989, 34: 1131–1136.
Huovinen S, Huovinen P, Torniainen K, Jacoby GA Evaluation of plasmid-encoded beta-lactamase resistance. European Journal of Clinical Microbiology 1988, 10: 651–655.
Jones RN, Barry AL, Gavan TL, Washington AJ Suceptibility tests: microdilution and macrodilution broth procedures. In: Lennette EH, Balows A, Hausler WJ, Shadomy HJ (ed): Manual of clinical microbiology. American Society for Microbiology, Washington, DC, 1985, p. 972–977.
Huovinen S, Huovinen P, Jacoby GA Detection of plasmid-mediated beta-lactamases with DNA probes. Antimicrobial Agents and Chemotherapy 1988, 32: 175–179.
Feinberg AP, Vogelstein B A technique for radiolabelling DNA restriction endonuclease fragments to high specific activity. Analytical Biochemistry 1983, 132: 6–13.
Papanicolau GA, Medeiros AA, Jacoby GA Novel plasmid-mediated beta-lactamase (MIR-1) conferring resistance to oxyimino- and a-methoxy beta-lactams in clinical isolates ofKlebsiella pneumoniae. Antimicrobial Agents and Chemotherapy 1990, 34: 2200–2209.
Huovinen S Rapid isoelectric focusing of plasmidmediated beta-lactamases with Pharmacia Phast-System. Antimicrobial Agents and Chemotherapy 1988, 32: 1730–1732.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Arstila, T., Huovinen, S. & Huovinen, P. Analysis of beta-lactamase production in ampicillin-resistantEscherichia coli isolated from blood cultures 1983–1989. Eur. J. Clin. Microbiol. Infect. Dis. 10, 1068–1070 (1991). https://doi.org/10.1007/BF01984932
Issue Date:
DOI: https://doi.org/10.1007/BF01984932