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Problems in interpretation of piperacillin susceptibility of TEM-1 producingEscherichia coli in the disk diffusion test

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Abstract

The susceptibility of 455Escherichia coli blood culture isolate to piperacillin was tested with the disk diffusion test. The presence of different beta-lactamase genes in these strains was also studied using DNA hybridization. Of the TEM beta-lactamase producing isolates, 64 % (61/95) were interpreted as intermediately susceptible to piperacillin. Because piperacillin is hydrolyzed by TEM-type beta-lactamases, we suggest that the intermediate susceptibility category should be reduced or omitted in testing piperacillin susceptibility ofEscherichia coli isolates.

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References

  1. Olsson-Liljequist B, Dornbusch K, and the referensgruppen för antibiotikafrågor: Resistensbestämning av bakterier mot antibiotika. Referens- och Metodbok. Stockholm, 1990.

  2. National Committee for Clinical Laboratory Standards Performance standards for antimicrobial disk susceptibility tests. M2-A4. NCCLS, Villanova, PA, 1990.

    Google Scholar 

  3. Medeiros AA, Jacoby GA Beta-lactamase mediated resistance. In: Queener SF, Webber JA, Queener SW (ed): Beta-lactam antibiotics for clinical use. Marcel Dekker, New York, 1986, p. 49–84.

    Google Scholar 

  4. Huovinen P, Herva E, Katila M-L, Klossner M-L, Renkonen O-V, Toivanen P Reliability of disk diffusion method using semiconfluent growth in the determination of aminoglycoside resistance. Acta Pathologica Microbiologica and Immunologica Scandinavica (B) 1986, 94: 153–157.

    Google Scholar 

  5. Huovinen S, Huovinen P, Jacoby GA Detection of plasmid-mediated beta-lactamases with DNA probes. Antimicrobial Agents and Chemotherapy 1988, 32: 175–179.

    PubMed  Google Scholar 

  6. Feinberg AP, Vogelstein B A technique for radiolabeling DNA restriction endonuclease fragments to high specific activity. Analytical Biochemistry 1983, 132: 6–13.

    PubMed  Google Scholar 

  7. Huovinen S Plasmid-mediated β-lactamase resistance in gram-negative bacilli. Detection of β-lactamases by using DNA-hybridization and isoelectric focusing. Thesis. Turku University, Turku, 1988, p. 1–66.

    Google Scholar 

  8. Jacoby GA, Sutton L Beta-lactamases and beta-lactam resistance inEscherichia coli. Antimicrobial Agents and Chemotherapy 1985, 28: 703–705.

    PubMed  Google Scholar 

  9. Gerlach BA, Wiedemann B Tn3 as the molecular basis of ampicillin resistance inE. coli — an epidemiological study. Zentralblatt für Bakteriologie, Mikrobiologie und Hygiene (A) 1985, 260: 139–150.

    Google Scholar 

  10. Philippon A, Labia R, Jacoby GA Extended-spectrum beta-lactamases. Antimicrobial Agents and Chemotherapy 1989, 33: 1131–1136.

    PubMed  Google Scholar 

  11. Cooksey R, Swenson J, Clark N, Gay E, Thornsberry C Patterns and mechanisms of beta-lactam resistance among isolates ofEscherichia coli from hospitals in the United States. Antimicrobial Agents and Chemotherapy 1990, 34: 739–745.

    PubMed  Google Scholar 

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Authors and Affiliations

  1. Department of Medical Microbiology, Turku University, Finland

    T. Arstila

  2. Antimicrobial Research Unit, National Public Health Institute, Kiinamyllynkatu 13, 20520, Turku, Finland

    T. Arstila, S. Huovinen & P. Huovinen

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  1. T. Arstila
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  2. S. Huovinen
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  3. P. Huovinen
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Arstila, T., Huovinen, S. & Huovinen, P. Problems in interpretation of piperacillin susceptibility of TEM-1 producingEscherichia coli in the disk diffusion test. Eur. J. Clin. Microbiol. Infect. Dis. 10, 1066–1067 (1991). https://doi.org/10.1007/BF01984931

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