Abstract
Variation amongst strains ofHelicobacter pylori was examined by35S-methionine-labelled protein SDS-PAGE (Radio-PAGE), immunoblot and DNA fingerprinting techniques. These methods allowed discrimination amongst isolates and showed total correlation. Pig and baboon gastricHelicobacter pylori-like organisms were found to be very similar toHelicobacter pylori by both Radio-PAGE and immunoblotting, whereas aHelicobacter mustelae isolate was markedly different. TheHindIII restriction endonuclease digest patterns ofHelicobacter pylori DNA illustrated the considerable genomic variation of this organism. The Radio-PAGE and immunoblot typing methods both gave precise identification ofHelicobacter pylori strains, but Radio-PAGE was found to give higher resolution and represents a standardised universally applicable fingerprinting method forHelicobacter pylori.
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Clayton, C.L., Kleanthous, H., Dent, J.C. et al. Evaluation of fingerprinting methods for identification ofHelicobacter pylori strains. Eur. J. Clin. Microbiol. Infect. Dis. 10, 1040–1047 (1991). https://doi.org/10.1007/BF01984926
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DOI: https://doi.org/10.1007/BF01984926