Agents and Actions

, Volume 40, Issue 3–4, pp 215–219

Cartilage destruction and osteophytes in instability-induced murine osteoarthritis: Role of TGFβ in osteophyte formation?

  • Wim B. van den Berg
  • Gerjo J. V. M. van Osch
  • Peter M. van der Kraan
  • Henk M. van Beuningen
Bone and Cartilage

Abstract

Osteoarthritis is characterized by focal cartilage destruction and marked formation of osteophytes. We have investigated the possible relationship between site specific occurrence of cartilage damage and osteophytes in the collagenase induced murine osteoarthritis model. The degree of instability of the joint correlated with the amount of cartilage loss. Moreover, cartilage damage in the medial tibial plateau correlated only strongly with the osteophyte at the medial plateau, whereas a similar, site directed trend was noted for lateral damage and lateral osteophytes. A separate study with intraarticular injection of TGFβ1 in normal murine knee joints revealed that this factor can induce osteophytes at characteristic sites, suggesting a role of endogenous TGFβ in this phenomenon.

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. [1]
    R. C. Thompson, Jr, T. R. Oegema, Jr., J. L. Lewis and L. Wallace,Osteoarthritic changes after acute transarticular load: An animal model. J. Bone Joint Surg.73A, 990–1001 (1991).Google Scholar
  2. [2]
    P. M. van der Kraan, E. L. Vitters, H. M. van Beuningen, L. B. A. van den Putte, W. B. van den Berg,Degenerative knee joint lesions in mice after a single intra-articular collagenase injection. A new model of osteoarthritis. J. Exp. Pathol.71, 19–31 (1990).Google Scholar
  3. [3]
    K. Brandt,Transection of the anterior cruciate ligament in the dog. A model of osteoarthritis. Semin. Arthritis Rheum.21, 22–32 (1991).CrossRefPubMedGoogle Scholar
  4. [4]
    G.J.V.M. van Osch, P.M. van der Kraan, E.L. Vitters, L. Blankevoort, W.B. van den Berg,Induction of osteoarthritis by intra-articular injection of collagenase in mice. Strain and sex related differences. Osteoarthritis and Cartilage1, 171–177 (1993).PubMedGoogle Scholar
  5. [5]
    G.R. Dodge, A.R. Poole,Immunohistochemical detection and immunochemical analysis of type II collagen degradation in human normal, rheumatoid and osteoarthritic articular cartilage and in explants of bovine articular cartilage with interleukin 1. J. Clin. Invest.83, 647–661 (1989).PubMedGoogle Scholar
  6. [6]
    P.M. van der Kraan, E.L. Vitters, H.M. van Beuningen, W.B. van den Berg,Proteoglycan synthesis and osteophyte formation in “metabolically” and “mechanically” induced murine degenerative joint disease: An in-vivo autoradiographic study. Int. J. Exp. Pathol.73, 335–350 (1992).PubMedGoogle Scholar
  7. [7]
    G. Wilhelmi, R. Maier,Beobachtungen über den Einfluss von Druckbelastung und Bewegung auf die Gelenke an Modell Arthrose-disponierter Mäuse. Akt. Rhematol,12, 161–167 (1987).Google Scholar
  8. [8]
    M.W.M. Kruijsen, W.B. van den Berg, L.B.A. van de Putte,Sequential alterations of periarticular structures in antigeninduced arthritis in mice. Histological observations on fibrous capsule, ligaments, bone and muscles, using whole joint sections. Brit. J. Exp. Pathol.64, 298–305 (1983).Google Scholar
  9. [9]
    B.J. de Vries, W.B. van den Berg,Impact of NSAIDs on murine antigen induced arthritis. II. A light microscopic investigation of antiinflammatory and bone protective effects. J. Rheumatol.17, 295–303 (1990).PubMedGoogle Scholar
  10. [10]
    M.E. Joyce, A.B. Roberts, M.B. Sporn, M.E. Bolander,Transforming growth factor-β and the initiation of chondrogenesis and osteogenesis in the rat femur. J. Cell. Biol.110, 2195–2207 (1990).CrossRefPubMedGoogle Scholar
  11. [11]
    H.M. van Beuningen, P.M. van der Kraan, O.J. Arntz, W.B. van der Berg,Transforming growth factor-β1 stimulates articular chondrocyte proteoglycan synthesis and induces osteophyte formation in the murine knee joint. Lab. Invest. in press.Google Scholar
  12. [12]
    H.M. van Beuningen, P.M. van der Kraan, O.J. Arntz, W.B. van den Berg,Protection against Interleukin-1-induced articular cartilage damage by transforming growth factor-β1 in vivo: Age-related differences. Am. J. Pathol. submitted.Google Scholar

Copyright information

© Birkhäuser Verlag 1993

Authors and Affiliations

  • Wim B. van den Berg
    • 1
  • Gerjo J. V. M. van Osch
    • 1
  • Peter M. van der Kraan
    • 1
  • Henk M. van Beuningen
    • 1
  1. 1.Department of RheumatologyUniversity Hospital NijmegenNijmegenThe Netherlands

Personalised recommendations