Helgoländer Meeresuntersuchungen

, Volume 34, Issue 1, pp 1–14 | Cite as

A method for the detection of bacteriophages from ocean water

  • K. Moebus


A method for the isolation of bacteriophages from ocean water is described. It precludes sample storage before starting phage-enrichment cultures and provides for the use of 3 sub-samples enriched with organic nutrients after 1, 2 and 3 days of incubation. The method was used with samples collected from 6 m below the surface at 48 stations between the European continental shelf and the Sargasso Sea. With 213 among 931 bacterial isolates about 250 strains of bacteriophages were detected by two methods of different sensitivity. From 14 samples taken east of the Azores 115 host bacteria have been found versus only 98 from 34 samples collected at westerly stations. The employment of more than one sub-sample per station as well as the use of more sensitive phage-detection procedures was found to be more advantageous the lower the concentration of cultivatable bacteria in a sample.


Waste Water Water Management Water Pollution Continental Shelf Bacterial Isolate 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Literature cited

  1. Chaina, P. N., 1965. Some recent studies on marine bacteriophages. — J. gen. Microbiol.41, Proc. 25.Google Scholar
  2. Hidaka, T., 1971. Isolation of marine bacteriophages from sea water. — Bull. Jap. Soc. scient. Fish.37, 1199–1206.Google Scholar
  3. Hidaka, T., 1977. Detection and isolation of marine bacteriophage systems in the southwestern part of the Pacific Ocean. — Mem. Fac. Fish., Kagoshima Univ.26, 55–62.Google Scholar
  4. Johnson, P. W. & Sieburth, J. McN., 1978. Morphology of non-cultured bacterioplankton from estuarine, shelf and open ocean waters. — Abstr. a. Meet. Am. Soc. Microbiol.N 95, 178.Google Scholar
  5. Spencer, R., 1955. A marine bacteriophage. — Nature, Lond.175, 690.Google Scholar
  6. Spencer, R., 1960. Indigenous marine bacteriophages. — J. Bact.79, 614.Google Scholar
  7. ZoBell, C. E., 1946. Marine microbiology. Chronica Botanica Comp., Waltham, Mass., 240 pp.Google Scholar

Copyright information

© Biologische Anstalt Helgoland 1980

Authors and Affiliations

  • K. Moebus
    • 1
  1. 1.Biologische Anstalt Helgoland (Meeresstation)HelgolandFederal Republic of Germany

Personalised recommendations