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, Volume 42, Issue 3–4, pp 101–106 | Cite as

[Ca2+]i-transients and actin polymerization in human neutrophils under stimulation with GROα and complement fragment C5a

  • Beatrix Metzner
  • Jörn Elsner
  • Gustav Dobos
  • Eckhard Kownatzki
  • Frauke Parlow
  • Ingrid Schraufstätter
  • Johannes Norgauer
Inflammation

Abstract

The neutrophil chemotaxins, complement fragment C5a (C5a) and GROα, induced the mobilization of Ca2+ from intracellular stores and the polymerization of actin in human neutrophils as assayed by flow cytometric measurements. [Ca2+]i-transients developed as an “all-or-none” response. Individual neutrophils required different threshold concentrations of added ligand to induce [Ca2+]i-transients which were then always maximal. In contrast, chemotaxin-induced formation of actin filaments in single neutrophils occurred in a dose-dependent manner. Pertussis toxin blocked chemotaxin-induced actin polymerization and [Ca2+]i-transients indicating that both cell responses shared initial activation steps such as ligand binding and activation of guanine nucleotide-binding proteins (G-proteins).

Key words

GROα Complement fragment C5a Actin Ca2+-transients 

Abbreviations

[Ca2+]i

Cytosolic free Ca2+

C5a

Complement fragment C5a

PtdlnsP2

Phosphatidylinositol (4,5)-bisphosphate

IP3

Inositol-trisphosphate

fluo-3

fluo-3-acethoxymethyl ester

NBD-phallacidin

7-nitrobenz-2-oxa-1,3-diazol-phallacidin

EGTA

[(2-(aminoethyl-glycolether-N,N,N′,N′-tetraacidic acid]

f-actin

filament actin

PT

pertussis toxin

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Copyright information

© Birkhäuser Verlag 1994

Authors and Affiliations

  • Beatrix Metzner
    • 1
  • Jörn Elsner
    • 1
  • Gustav Dobos
    • 1
  • Eckhard Kownatzki
    • 1
  • Frauke Parlow
    • 1
  • Ingrid Schraufstätter
    • 2
  • Johannes Norgauer
    • 1
  1. 1.Department of DermatologyUniversity of FreiburgFreiburgGermany
  2. 2.Department of ImmunologyThe Scripps Research InstituteLa JollaUSA

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