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, Volume 32, Issue 1–2, pp 41–45 | Cite as

Adherence-induced enhancement of the oxidative burst of human neutrophilic granulocytes: Effects of the surface coat and of divalent cations

  • E. Kownatzki
  • S. Uhrich
Papers Given at the 12th European Workshop on Inflammation, Halle, Germany, May 30–31, 1990 Topical Research Problems

Abstract

Neutrophilic granulocytes are capable of adhering to biological or artificial surfaces. In addition to specific interactions between adherence molecules on the cell surface and corresponding structures on other cells or matrix proteins like fibronection or collagen there appeared to be non-specific attachment as well. Adherence augmentation induced by stimulation with chemotactic factors or cytokines was an active process which did not proceed at 4°C and after removal of divalent cations from the medium. Adherence acted as a priming stimulus increasing the amount of superoxide anion and hydrogen peroxide produced in response to stimulation by certain chemotactic factors and cytokines. Adherence priming like priming by GM-CSF was strongly suppressed by chelation of intracellular Ca2+ ions. The two priming mechanisms differed, however, in their requirement for divalent cations in the external medium: whereas Mg2+ suppressed GM-CSF priming, it synergised with Ca2+ in the adherence-augmented oxidative burst.

Keywords

Hydrogen Peroxide Cell Surface Superoxide Adherence Molecule Active Process 

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Copyright information

© Birkhäuser Verlag 1991

Authors and Affiliations

  • E. Kownatzki
    • 1
  • S. Uhrich
    • 1
  1. 1.Division of Experimental Dermatology, Department of DermatologyUniversity of FreiburgFRG

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