Plant Cell, Tissue and Organ Culture

, Volume 33, Issue 2, pp 203–210 | Cite as

Plant regeneration fromin vitro leaf culture of severalGerbera species

  • Jean-Paul Reynoird
  • Dominique Chriqui
  • Michèle Noin
  • Spencer Brown
  • Dominique Marie
Article

Abstract

Efficient bud regeneration was obtained from a clone ofGerbera hybrida Bol. L. leaf explants cultured on modified Murashige and Skoog medium supplemented with 10 µM benzyladenine and 2.5 µM naphthalenacetic acid. The morphogenic potential varied with the developmental stage of the leaves. Up to 90% of excised developing leaves formed 3 to 5 shoots per explant. Bud regeneration was not obtained on the same medium with fully expanded leaves. Addition of 0.05 µM or 0.5 µM thidiazuron to the above medium significantly promoted regeneration from mature leaves but was ineffective for similar explants of a recalcitrant clone. The two wild speciesG. viridifolia Schultz Bip. andG. piloselloides L. Cass. also displayed specific multiplication habits and regeneration abilities. Bud regeneration occurred from callus. Chromosome counts and DNA flow cytometry indicated that all the regenerants were typically diploid, as were the various tissues of the mother plants. Afterin vitro rooting and acclimatization, no phenotypic variations were detected among the regenerants during both their vegetative and reproductive phases.

Key words

DNA flow cytometry growth regulator effects thidiazuron 

Abbreviations

BA

benzyladenine

NAA

α-naphthalenacetic acid

TDZ

thidiazuron

IAA

indoleacetic acid

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Copyright information

© Kluwer Academic Publishers 1993

Authors and Affiliations

  • Jean-Paul Reynoird
    • 1
  • Dominique Chriqui
    • 2
  • Michèle Noin
    • 2
  • Spencer Brown
    • 3
  • Dominique Marie
    • 3
  1. 1.Francereco S.A., Centre de Biotechnologie VégétaleNotre Dame d'OéFrance
  2. 2.Laboratoire C.E.M.V., Bât. N2Université Pierre et Marie CurieParis Cedex 05France
  3. 3.Service de CytométrieInstitut des Sciences Végétales, C.N.R.S.Gif-sur-YvetteFrance

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