Advertisement

Agents and Actions

, Volume 16, Issue 3–4, pp 287–290 | Cite as

Evidence of mast-cell histamine being mitogenic in intact tissue

  • Klas Norrby
Histamine in Pathological States

Abstract

We have reported previously that secretion by connective tissue mast cells (MCs) causes mitogenesis in adjacent cells in diverse rat tissues.

In cultured rat mesentery there was a spontaneous release of about 45% of the histamine in 2 days, and a spontaneous marked increase in basal proliferation of the mesentery. The MC secretagogues, compound 48/80 and polymyxin B, released additional histamine and stimulated mitogenesis further. In contrast, 48/80 added to cultures of guinea-pig mesentery, the MC of which are unresponsive to the drug, did not affect the basal proliferation. However, exogenous histamine at 10−10M mitogenically stimulated the cultured guinea-pig mesentery. A histmaine H2-receptor antagonist, which itself was mitogenically inert, significantly suppressed the 48/80-induced MC-mediated mitogenesis in rat mesenteryin vivo andin vitro. On the other hand, a histamine H1-receptor antagonist did not affect this MC-mediated mitogenesis in rat.

Our findings indicate that histamine is one of possibly several mitogens which are released or activated by the secreting MC.

Keywords

Mast Cell Connective Tissue Histamine Adjacent Cell Polymyxin 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. [1]
    R.W. Schayer, Enzymatic formation of histamine from histidine. InHandbook of Experimental Pharmacology, vol. 18, part 1 (Ed.L. Rocha &Silva). Springer, Berlin 1966.Google Scholar
  2. [2]
    G. Kahlson andE. Rosengren,New approaches in the physiology of histamine, Physiol. Rev.48, 155–196 (1968).PubMedGoogle Scholar
  3. [3]
    K. Norrby, L. Enerbäck andL. Franzen,Mast cell activation and tissue cell proliferation, Cell Tiss. Res.170, 289–303 (1976).CrossRefGoogle Scholar
  4. [4]
    L. Franzen andK. Norrby,Local mitogenic effect of tissue mast cell secretion, Cell Tiss. Kinet.13, 635–642 (1980).Google Scholar
  5. [5]
    K. Norrby,On the 48/80-induced secretion of tissue mast cells and its mitogenic effect on nearby cells in the intact rat, Virchows Arch. path. Anat. Physiol.38, 57–65 (1981).Google Scholar
  6. [6]
    K. Norrby,Intradermal mast-cell secretion causing cutaneous mitogenesis, Virchows Arch. path. Anat. Physiol.42, 263–269 (1983).Google Scholar
  7. [7]
    K. Norrby andS. Eneström,Cellular and extracellular changes following mast-cell secretion in avascular rat mesentery. An electron-microscopic study, Cell Tiss. Res.235, 339–345 (1984).CrossRefGoogle Scholar
  8. [8]
    D.D. Metcalfe,Effector cell heterogeneity in immediate hypersensitivity reactions, Clin. Rev. Allergy1, 311–325 (1983).PubMedGoogle Scholar
  9. [9]
    L. Franzen andK. Norrby,Immunological challenge causes mitogenic stimulation in normal connective tissue cells, Acta path. microbiol. scand.90, 385–389 (1982).Google Scholar
  10. [10]
    K. Norrby,Effect of heparin, histamine and serotonin on the density-dependent inhibition of replication in two fibroblastic cell lines, Virchows Arch. path. Anat. Physiol.15, 75–93 (1973).Google Scholar

Copyright information

© Birkhäuser Verlag 1985

Authors and Affiliations

  • Klas Norrby
    • 1
  1. 1.Department of PathologyUniversity of LinköpingSweden

Personalised recommendations