Applied Microbiology and Biotechnology

, Volume 29, Issue 2–3, pp 181–185 | Cite as

Physiological control and process kinetics of clavine alkaloid production byClaviceps purpurea

  • Miroslav Flieger
  • Jaroslav Votruba
  • Vladimír Křen
  • Sylvie Pažoutová
  • Viktor Rylko
  • Přemysl Sajdl
  • Zdeněk Reháček


Kinetic parameters of production of clavine alkaloids were evaluated in twoClaviceps purpurea strains. Mutagenesis brought about enhanced resistance of the biosynthetic system towards alkaloids. Addition of glucose into the fermentation medium altered the zero order kinetics of production to activation-inhibition kinetics. The glucose treatment allowed performance of both “elymoclavine-inhibitionless” and “clavine alkaloid-decompositionless” fermentations if a combination of fermentation and separation units in a closed loop was used.


Glucose Fermentation Alkaloid Kinetic Parameter Closed Loop 
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rate constant of agroclavine synthesis (mg Agro · mg Elymo/l·g DW·day for stage 1, mg Agro/g DW·day for stage 2)


parameter describing inhibition of agroclavine formation rate by elymoclavine (mg Elymo/l)


specific rate of agroclavine decay (l/g DW·day)


maximal specific rate of elymoclavine synthesis (stage 1, 1/g DW·day, stage 2, mg Elymo/g DW·day)


maximal specific rate of elymoclavine synthesis in stage 1 (inhibition-activation mechanism) (mg Elymo/g DW·day)


physiological constant describing the elymoclavine decay rate (l2/g DW·day·mg Elymo)


physiological constant describing the activation of elymoclavine biosynthesis by elymoclavine (mg Elymo/l)


physiological constant describing the repression of elymoclavine biosynthesis by elymoclavine (mg Elymo/l)


maximal specific growth rate (1/day)


specific rate of biomass decay (l/g DW·day)


agroclavine concentration (mg/l)


elymoclavine concentration (mg/l)


specific rate of agroclavine biosynthesis (mg Agro/g DW·day)


specific rate of elymoclavine biosynthesis (mg Elymo/g DW·day)


specific rate of alkaloid biosynthesis (mg alkaloid/g DW·day)


dry biomass concentration (g/l)


specific growth rate (1/day)









dry weight of biomass


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Copyright information

© Springer-Verlag 1988

Authors and Affiliations

  • Miroslav Flieger
    • 1
  • Jaroslav Votruba
    • 1
  • Vladimír Křen
    • 1
  • Sylvie Pažoutová
    • 1
  • Viktor Rylko
    • 1
  • Přemysl Sajdl
    • 1
  • Zdeněk Reháček
    • 1
  1. 1.Institute of MicrobiologyCzechoslovak Academy of SciencesPrague 4Czechoslovakia

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