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Applied Microbiology and Biotechnology

, Volume 29, Issue 2–3, pp 113–118 | Cite as

Oxygen uptake rate of immobilized growing hybridoma cells

  • Yoshihito Shirai
  • Kenji Hashimoto
  • Hideki Yamaji
  • Hiroyuki Kawahara
Biotechnology

Summary

The specific oxygen uptake rate of hybridoma cells immobilized in calcium alginate gel particles was measured, and the observed data was compared with those of non-immobilized cells. The uptake rate of the immobilized cells coincided with that of the non-immobilized hybridoma cells just after immobilization, but increased with cell growth. On the other hand, the cellular glucose consumption rate decreased slightly during the experiments. The increased oxygen uptake rate by immobilized cells was closely related to the formation of cell colonies in the gel particles.

Keywords

Immobilization Alginate Uptake Rate Immobilize Cell Glucose Consumption 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer-Verlag 1988

Authors and Affiliations

  • Yoshihito Shirai
    • 1
  • Kenji Hashimoto
    • 1
  • Hideki Yamaji
    • 1
  • Hiroyuki Kawahara
    • 1
  1. 1.Department of Chemical Engineering, Faculty of EngineeringKyoto UniversityKyotoJapan

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