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The effect of calcium on cardiac anaphylaxis in guinea-pig Langendorff heart preparations

  • Immunosuppression and Inflammation
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Abstract

This study was designed to determine the effects of different calcium concentrations on the perfused isolated guinea-pig heart preparation subjected to cardiac anaphylaxis. Following challenge both physiological and biochemical effects were determined on hearts from guinea-pigs previously sensitized to ovalbumin. Perfusion media containing either 1, 2.54 or 5 mM of calcium was used.

In comparison to nonsensitized controls challenged to ovalbumin, challenged sensitized hearts (CSH) perfused with 1 mM Ca2+ showed an initial increase indF/dt, a prolonged rise in H.R. and depressed coronary flow. Raising the calcium concentration to either 2.54 or 5 mM in CSH preparations resulted in a marked increase in the release of lactate dehydrogenase (LDH) into the coronary effluent and depressed coronary flow. Perfusing CSH preparations with increasingly higher calcium concentrations more often produced severe tachyarrhythmias and fibrillation. The highest level of histamine released into the coronary effluent occurred immediately following challenge and then declined exponentially over the next 20 min. Both challenge and the administration of histamine induced an immediate but transient increase in H.R., a rise indF/dt, and LDH release. The infusion of histamine produced an increase in coronary flow, but on porcine tubular coronary arterial segments only a direct constricting effect was obtained. The prior administration of cimetidine (10−5 M) attenuated the rise in LDH anddF/dt in CSH and nonsensitized preparations infused with histamine (3 μg). However, although cimetidine did not affect the decreased coronary flow in CSH preparations, it initially attenuated the rise in coronary flow in preparations infused with histamine.

These results suggest that calcium enhances the liklihood of tachyarrhythmias in cardiac anaphylaxis. The release of LDH in histamine-infused preparations and those CSH preparations perfused with 2.54 and 5 mM calcium-containing medias also suggests the possibility that calcium enhances the damaging effects on the myocardial cell in cardiac anaphylaxis.

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References

  1. J.L. Waller, J.A. Kaplan andE.L. Jones, Anesthesia for coronary revascularization. InCardiac Anesthesia, pp. 266–268. (Ed.J.A. Kaplan). Grune and Stratton, New York, NY 1979.

    Google Scholar 

  2. D.C. Finlayson, Postoperative intensive care. InCardiac Anesthesia, pp. 473–474. (Ed. J.A. Kaplan). Grune and Stratton, New York, NY 1979.

    Google Scholar 

  3. S.I. Wasserman,Mediators of immediate hypersensitivity, J. Allergy Clin. Immunol.72, 101–115 (1983).

    Article  PubMed  Google Scholar 

  4. R.D. Tanz, N.J. Russell, S.P. Banerian andV.H. Sharp,Ouabain-induced tacharrhythmias and cell damage in isolated perfused guinea-pig hearts: I. protection by propranolol, J. Mol. Cell. Cardiol.14, 655–671 (1982).

    Article  PubMed  Google Scholar 

  5. F. Wroblewski andJ.S. LaDue,Lactic dehydrogenase activity in blood, Proc. Soc. exp. Biol. Med.90, 210–213 (1955).

    PubMed  Google Scholar 

  6. R.P. Sirganian. Histamine release and assay methods for the study of human allergy. InManual of Clinical Immunology, pp. 603–615. (EdsN.R. Rose andH. Friedman). Amer. Soc. for Microbiology. Washington, D.C 1976.

    Google Scholar 

  7. R.D. Tanz, T. Heskett, R.W. Loehning andC.A. Fairfax,Comparative cardiotoxicity of bupivacaine and lidocaine in the isolated perfused mammalian heart, Anesth. Analg. curr. Res.63, 549–556 (1984).

    Google Scholar 

  8. R. Levi, J.R. Malm, F.O. Bowman andM.R. Rosen,The arrhythmogenic actions of histamine on human atrial fibers, Circulation Res.49, 545–550 (1981).

    PubMed  Google Scholar 

  9. R. Levi,Effects of exogenous and immunologically released histamine on the isolated heart: a quantitative comparison, J. Pharmac. exp. Ther.182, 227–238 (1972).

    Google Scholar 

  10. W. Flacke, D. Atanacković, R.A. Gillis andM.H. Alper,The actions of histamine on the mammalian heart J. Pharmac. exp. Ther.155, 271–278 (1967).

    Google Scholar 

  11. W.C. Bowman andM.J. Rand,Textbook of Pharmacology, 2nd ed., pp. 22–26. Blackwell Scientific Publications, Oxford 1980.

    Google Scholar 

  12. R. Levi andA.J. Pappano,Modification of the effects of histamine and norepinephrine on the sinoatrial node pacemaker by potassium and calcium, J. Pharmac. exp. Ther.204, 625–633 (1978).

    Google Scholar 

  13. A.L. Bartlet,The action of histamine on the isolated heart, Br. J. Pharmac.21, 450–461 (1963).

    Google Scholar 

  14. H.G. Barbour,Note on the action of histamin upon surviving arteries, J. Pharmac. exp. Ther.4, 245–250 (1913).

    Google Scholar 

  15. K.J. Broadley,The role of H 1-and H 2-receptors in the coronary vascular response to histamine of isolated perfused hearts of guinea-pigs and rabbits, Br. J. Pharmac.54, 511–521 (1975).

    Google Scholar 

  16. S.B. Flynn, R.W. Gristwood andD.A.A. Owen,Differentiation of the roles of histamine H 1-and H 2-receptors in the mediation of the effects of histamine in the isolated working heart of the guinea-pig, Br. J. Pharmac.65, 127–137 (1979).

    Google Scholar 

  17. J.A. Gunn,The action of histamine on the heart and coronary vessels, J. Pharmac. exp. Ther.29, 325–337 (1926).

    Google Scholar 

  18. H.B. Wilcox andE.C. Andrus,Anaphylaxis in the isolated heart, J. exp. Med.67, 169–180 (1938).

    Article  Google Scholar 

  19. G.A. Feigen, E.M. Vaughan Williams, J.K. Peterson andC.B. Nielsen,Histamine release and intracellular potentials during anaphylaxis in the isolated heart, Circulation Res.8, 713–723 (1960).

    PubMed  Google Scholar 

  20. A.G. Gilman, L.S. Goodman andA. Gilman,The Pharmacological Basis of Therapeutics, 6th ed. (EdsA.G. Gilman,L.S. Goodman andA. Gilman). Macmillan Pub. Co., N.Y. 1980.

    Google Scholar 

  21. R. Levi, J.A. Burke andE.J. Corey, SRS-A, leukotrienes and immediate hypersensitivity reactions of the heart. InLeukotrienes and Other Lipoxygenase Products, pp. 215–222. (EdsB. Samuelsson andR. Paoletti). Raven Press, New York, NY 1982.

    Google Scholar 

  22. J.H. Zavecz andR. Levi,Histamine-induced negative inotropism: mediation by H 1-receptors, J. Pharmac. exp. Ther.206, 274–280 (1978).

    Google Scholar 

  23. R.W. Gristwood, J.C.R. Lincoln andD.A.A. Owen,Effects of histamine on human isolated heart muscle: comparison with effects of noradrenaline, J. Pharm. Pharmac.32, 145–146 (1980).

    Google Scholar 

  24. U. Trendelenburg,The action of histamine and 5-hydroxytryptamine on isolated mammalian atria, J. Pharmac. exp. Ther.130, 450–460 (1960).

    Google Scholar 

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Authors and Affiliations

  1. Department of Pharmacology and Department of Anesthesiology, The Oregon Health Sciences University, 97201, Portland, Oregon, USA

    Ralph D. Tanz, Nancy Kettelkamp & Carol A. Hirshman

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  1. Ralph D. Tanz
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  2. Nancy Kettelkamp
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  3. Carol A. Hirshman
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Additional information

This study was supported by grants from the G. and L. Pfeiffer Medical Research Foundation, the Oregon Heart Association and NIH HL-25831. The authors wish to acknowledge the excellent technical assistance of Mr Dan Austin, and the use of equipment in the laboratory of Dr J.M. Hanifin.

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Tanz, R.D., Kettelkamp, N. & Hirshman, C.A. The effect of calcium on cardiac anaphylaxis in guinea-pig Langendorff heart preparations. Agents and Actions 16, 415–424 (1985). https://doi.org/10.1007/BF01982883

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  • DOI: https://doi.org/10.1007/BF01982883

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