Abstract
The content of 5-hydroxytryptamine (5HT) in various tissues of mutant mouse (W/W v) deficient in mast cells and of control mouse (+/+) was determined by high performance liquid chromatography. The depletion of mast cells in the mutant mouse (W/W v) was expected to cause a decrease in the 5HT content. In the control mice, 5HT was most densely accumulated in the lung (9.66±5.23 νg/g). Large intestine (6.40±2.61 μg/g) and stomach (6.10±2.14 μg/g) followed the lung in the rating of the 5HT content. The 5HT content ofW/W v mice was only 23.4% and 4.1% that of the control in the stomach (p<0.01) and the skin (p<0.01), respectively. The results were consistent with the expectation. In other organs (small intestine, caecum, large intestine, brain, lung, blood and salivary gland), the difference between theW/W v and normal mice was not statistically significant. The difference in the 5HT content of the stomach between the two genotypes was 4.67 μg/g and was much larger than the 5HT content (0.49μg/g) of normal mouse skin. With regard to the relatively small number of mast cells present in the stomach, the great difference in the 5HT content in the stomach between the two genotypes cannot be explained by the loss of mast cells. Hence, besides mast cells other cells may contribute to the high 5HT content of the stomach.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
W. Rubin, M.D. Gershon andL.L. Ross,Electron microscope radioautographic identification of serotonin-synthesizing cells in the mouse gastric mucosa, J. Cell Biol.50, 399–415 (1971).
H. Inokuchi, K. Kawai, Y. Takeuchi andY. Sano,Immunohistochemical demonstration of EC cells in rat gastrointestinal tract, Histochemistry74, 453–456 (1982).
M. Osaka, T. Fujita andY. Yanatori,On the possible role of intestinal hormones as the diarrhoeagenic messenger in cholera, Virchows Arch. B. Cell Path.18, 287–296 (1975).
H. Ahlman, H.N. Bhargava, P.E. Donahue, B. Newson, T.K. Das Gupta andL.M. Nyhus,The vagal release of 5-hydroxytryptamine from enterochromaffin cells in the cat, Acta Physiol. Scand.104, 262–270 (1978).
S. Takahashi andR. Yui,Gastrin-releasing peptide (GRP) and serotonin in the human fetal lung: an immunohistochemical study, Biomedical Res.4, 315–320 (1983).
J.M. Lauweryns, V. de Bock, A.A.J. Verhofstad andH.W.M. Steinbusch,Immunohistochemical localization of serotonin in intrapulmonary neuroepithelial bodies, Cell Tiss. Res.226, 215–223 (1982).
M. Yanagisawa, H. Hasegawa, A. Ichiyama, S. Hosoda andW. Nakamura,Comparison of serotonin-producing murine mastocytomas, P-815 and FMA3: determination of tryptophan hydroxylase, aromatic l-amino acid decarboxylase, and cellular concentration of tryptophan, 5-hydroxytryptophan, 5-hydroxytryptamine and 5-hydroxyindoleacetic acid, Biomedical Res.5, 19–28 (1984).
W.K. Silvers, A model for mammalian gene action and interaction. InThe Coat Color of Mice, pp. 206–223. Springer-Verlag, New York 1979.
Y. Kitamura, S. Go andK. Hatanaka,Decrease of mast cells in W/W v mice and their increase by bone marrow transplantation, Blood52, 447–452 (1978).
A. Yamatodani, K. Maeyama, T. Watanabe, H. Wada andY. Kitamura,Tissue distribution of histamine in a mutant mouse deficient in mast cells: clear evidence for the presence of non-mast cell histamine, Biochem. Pharmacol.31, 305–309 (1982).
A.H. Straus, H.B. Nader andC.P. Dietrich,Absence of heparin or heparin-like compounds in mast-cell-free tissues and animals, Biochim. Biophys. Acta717, 478–485 (1982).
J.M. Lauweryns, M. Cokelaere, T. Lerut andP. Theunynck,Cross circulation studies on the influence of hypoxia and hypoxaemia on neuro-epithelial bodies in young rabbits, Cell Tiss. Res.193, 373–386 (1978).
F.L. Pearce,Functional heterogeneity of mast cells from different species and tissues, Klin. Wochenschr.60, 954–957 (1982).
P.K. Crowle andN.D. Reed,Rejection of the intestinal parasite Nippostrongylus brasiliensis by mast cell-deficient W/W v anemic mice, Infect. Immun.33, 54–58 (1981).
U. Wingren, L. Enerbäck, H. Ahlman, S. Allenmark andA. Dahlström,Amines of the mucosal mast cell of the gut in normal and nematode infected rats, Histochemistry77, 145–158 (1983).
M. Shimada, K. Kitamura, M. Yokoyama, Y. Miyano, K. Maeyama, A. Yamatodani, Y. Takahashi andM. Tatsuta,Spontaneous stomach ulcer in genetically mast-cell depleted W/W v mice, Nature (London),283, 662–664 (1980).
Author information
Authors and Affiliations
Additional information
This work was supported in part by grants from Takeda Science Foundation (1982), from the Ministry of Education, Science and Culture of Japan (Grant-in-Aid for Special Project Research. 1981–83) and from National Center of Nervous, Mental and Muscular Disorders (NCNMMD) of the Ministry of Health and Welfare, Japan (1981–83).
To whom correspondence should be addressed
Rights and permissions
About this article
Cite this article
Inoue, F., Hasegawa, H., Nishimura, M. et al. Distribution of 5-hydroxytryptamine (5HT) in tissue of a mutant mouse deficient in mast cell (W/W v). Demonstration of the contribution of mast cells to the 5HT content in various organs. Agents and Actions 16, 295–301 (1985). https://doi.org/10.1007/BF01982861
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF01982861