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Vasoregulin, a glucocorticoid-inducible vascular permeability inhibitory protein

  • Corticosteroid Actions in Infalmmation
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Abstract

A vascular permeability inhibitory protein ‘Vasoregulin’ was induced by dexamethasone and other glucocorticoids in Namalva cells. Precipitates produced by trichloroacetic acid from cultured supernatant (crude released vasoregulin) suppressed serotonin-induced paw edema of mice (ID30=750 μg/mouse) when dosed 30 min. before serotonin. Carrageenan paw edema of rats was also suppressed (ID30 =9 mg/rat, s.c. and i.p., not by oral dosing) when the compound was injected at the same time as carrageenan and the edema determined after 3 hrs. The possible effect of glucocorticoid itself being contained in the vasoregulin preparation was excluded. The presence of Cu, Zn-superoxide dismutase (SOD), spermidine and spermine also gave good yields of vasoregulin in culture; SOD protected the inactivation of vasoregulin from superoxide radicalin vitro. Vasoregulin partially purified by Sephadex G-75 columns had no malonaldehyde-formation inhibitory capacity. Thus, vasoregulin is a glucocorticoid-inducible anti-inflammatory protein which differs from lipocortin.

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References

  1. G.J. Blackwell, R. Carnuccio, M. Di Rosa, R.J. Flower, L. Parente andP. Perscio,Macrocortin: A polypeptide causing the antiphospholipase effect of glucocorticoids, Nature287, 147–149 (1980).

    Article  PubMed  Google Scholar 

  2. F. Hirata, E. Schiffman, K. Venkatasubramanian, D. Salomon andJ. Axelrod,A phospholipase A 2 inhibitory protein in rabbit neutrophils induced by glucocorticoids, Natl. Acad. Sci. USA77, 2533–2536 (1980).

    PubMed  Google Scholar 

  3. F. Russo-Marie andD. Duval,Dexamethasone-induced inhibition of prostaglandin production does not result from a direct action on phospholipase activities but is mediated through a steroid-inducible factor, Biochem. Biophys. Acta712, 177–185 (1982).

    PubMed  Google Scholar 

  4. S. Turufuji, K. Sugio andF. Takanaka,The role of glucocorticoid receptor and gene expression in the anti-inflammatory action of dexamethasone, Nature280, 408–410 (1979).

    PubMed  Google Scholar 

  5. Y. Òyanagui,Steroid-like anti-inflammatory effect of superoxide dismutase in serotonin-, histamine- and kinin-induced edemata of mice: Existence of vascular permeability regulating protein(s), Biochem. Pharmacol.30, 1791–1798 (1981).

    Article  PubMed  Google Scholar 

  6. Y. Òyanagui,Anti-inflammatory effects of polyamines in serotonin and carrageenan paw edemata — possible mechanism to increase vascular permeability inhibitory protein level which is regulated by glucocorticoids and superoxide radical, Agents and Actions14, 228–237 (1984).

    Article  PubMed  Google Scholar 

  7. T. Oka andJ.W. Perry,Spermidine as a possible mediator of glucocorticoid effect on milk protein synthesis in mouse mammary epithelium in vitro, J. biol. Chem.249, 7647–7652 (1974).

    PubMed  Google Scholar 

  8. Y. Òyanagui,Physiological regulation of vascular permeability by endogenous glucocorticoids and active oxygen, Inflammation7, 81–89 (1983).

    Article  PubMed  Google Scholar 

  9. S. Glatt andY. Barenholz,Phospholipase A 1 from rat brain, specific for α′-position of lecithin, Methods in Enzymology vol. 14, (EdsS.P. Colowick andN.O. Kaplan, Academic Press) pp. 167–170 (1969).

  10. Y. Òyanagui,Inhibition of superoxide anion production in macrophages by anti-inflammatory drugs, Biochem. Pharmacol.25, 1473–1480 (1976).

    Article  PubMed  Google Scholar 

  11. Y. Òyanagui,Platonin, a photosensitizing cyanin dye, suppress acute inflammation, Arch. int. Pharmacodyn.266, 162–172 (1983).

    PubMed  Google Scholar 

  12. P. Ghiara, R. Meli, L. Parente andP. Persico,Distinct inhibition of membrane-bound and lysosomal phospholipase A 2 by glucocorticoid-induced proteins, Biochem. Pharmacol.33, 1445–1450 (1984).

    Article  PubMed  Google Scholar 

  13. P.D. Wightman, M.E. Dahlgren, P. Davies andR.J. Bonney,Identification and characterization of two phospholipase A 2 activities in resident mouse peritoneal macrophages, Biochem. J.195, 427–433 (1981).

    PubMed  Google Scholar 

  14. H. Araki, R.C. Peck, A.M. Lefer andJ.B. Smith,Mechanism of protection against arachidonate induced sudden death by glucocorticoid, Prostaglandins21, 387–399 (1981).

    Article  PubMed  Google Scholar 

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  1. Research Laboratories of Fujisawa Pharmaceutical Co., 1-6, 2-Chome, Kashima, Yodogawa-ku, 532, Osaka, Japan

    Yoshihiko Òyanagui & Shingo Suzuki

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  1. Yoshihiko Òyanagui
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  2. Shingo Suzuki
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Òyanagui, Y., Suzuki, S. Vasoregulin, a glucocorticoid-inducible vascular permeability inhibitory protein. Agents and Actions 17, 270–277 (1986). https://doi.org/10.1007/BF01982619

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  • DOI: https://doi.org/10.1007/BF01982619

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