Abstract
The colonization of hospitalized patients byPseudomonas aeruginosa at an intensive care unit for burn victims was studied over a one-year period. A total of 231 isolates from 12 patients were analyzed by macrorestriction analysis. The results revealed that most patients were infected with only one to three different strains. In several patientsPseudomonas aeruginosa isolates of the same clonal lineage exhibited considerable differences in their macrorestriction fragment pattern. Digestion with further restriction enzymes, however, allowed differentiation between clonal variants due to a high genetic drift and superinfection with a differentPseudomonas aeruginosa strain. Isolates of the same clonal lineage could be isolated from several patients as well as from the patients' environment. Notably,Pseudomonas aeruginosa could be isolated from sedimentation plates. Thus, patients may have been extensively cross-infected on the ward. These data underline the importance of strict infection control measures and of regular surveillance forPseudomonas aeruginosa by an appropriate typing method, i.e. one that can differentiate strains with high genomic variability.
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Bout TF, Welsh MJ, Beaudet AL Cystic fibrosis. In: Scriver CR, Beaudet AL, Sly WS, Valle D (ed): The metabolic basis of inherited disease. McGraw-Hill, New York, 1989, p. 2649–2680.
Mason AD, McManus AT, Pruitt BA Association of burn mortality and bacteremia: a 25-year review. Archives of Surgery 1986, 121: 1027–1031.
Sittig K, Deitch EA Effect of bacteremia on mortality after thermal injury. Archives of Surgery 1988, 123: 1367–1370.
McManus AT, Mason AD, McManus WF, Pruitt BA Twenty-five year review ofPseudomonas aeruginosa bacteremia in a burn center. European Journal of Clinical Microbiology and Infectious Diseases 1985, 4: 219–223.
Deitch EA The management of burns. New England Journal of Medicine 1990, 323: 1249–1253.
Grothues D, Koopmann U, van der Hardt H, Tümmler B Genome fingerprinting ofPseudomonas aeruginosa indicates colonization of cystic fibrosis siblings with closely related strains. Journal of Clinical Microbiology 1988, 26: 1973–1977.
Arbeit RD, Arthur M, Dunn R, Kim C, Selander RK, Goldstein R Resolution of recent evolutionary divergence amongEscherichia coli from related lineages: the application of pulsed field electrophoresis to molecular epidemiology. Journal of Infectious Diseases 1990, 161: 230–235.
Haertl R, Bandlow G Epidemiological typing ofEnterobacter cloacae by small-fragment restriction endonuclease analysis and pulsed-field gel electrophoresis of genomic restriction fragments. Journal of Clinical Microbiology 1993, 31: 128–133.
Murray BE, Singh KV, Heath JD, Sharma BR, Weinstock GM Comparison of genomic DNAs of different enterococcal isolates using restriction endonucleases with infrequent recognition sites. Journal of Clinical Microbiology 1990, 28: 2059–2063.
Hector JSR, Pang Y, Mazurek GH, Zhang Y, Brown BA, Wallace Jr JR Large restriction fragment patterns of genomicMycobacterium fortuitum DNA as strain-specific markers and their use in epidemiologic investigation of four nosocomial outbreaks. Journal of Clinical Microbiology 1992, 30: 1250–1255.
Goering RV, Duensing TD Rapid field inversion gel electrophoresis in combination with an rRNA probe in the epidemiological evaluation of staphylococci. Journal of Clinical Microbiology 1990, 28: 426–429.
Schlichting C, Branger C, Fournier JM, Witte W, Boutonnier A, Wolz C, Goullet P, Döring G Typing ofStaphylococcus aureus by pulsed-field gel electrophoresis, zymotyping, capsular typing, and phage typing: resolution of clonal relationships. Journal of Clinical Microbiology 1993, 31: 227–232.
Sahm DF, Washington DA Antibacterial susceptibility tests: dilution methods. In: Balows A, Hausler WJ, Herrmann KL, Isenberg HD, Shadomy, HJ (ed): Manual of clinical microbiology. American Society for Microbiology, Washington DC, 1991, p. 1105–1116.
Grothues D, Tümmler B Genome analysis ofPseudomonas aerugínosa by field inversion gel electrophoresis. FEMS Microbiology Letters 1987, 48: 419–422.
Bautsch W, Grothues D, Tümmler B Genome fingerprinting ofPseudomonas aeruginosa by two-dimensional field inversion gel electrophoresis. FEMS Microbiology Letters 1988, 52: 255–258.
Boukadida J, De Montalembert M, Gaillard JL, Gobin J, Grimont F, Girault D, Veron M, Berche P Outbreak of gut colonization byPseudomonas aeruginosa in immunocompromised children undergoing total digestive decontamination: analysis by pulsed-field electrophoresis. Journal of Clinical Microbiology 1991, 29: 2068–2071.
Alvarado CJ, Stolz SM, Maki DG Nosocomial infections from contaminated endoscopes: a flawed automated endoscope washer. An investigation using molecular epidemiology. American Journal of Medicine 1991, 91, Supplement 3B: 272–280.
Römling U, Grothues D, Koopmann U, Jahnke B, Greipel J, Tümmler B Pulsed-field gel electrophoresis analysis ofPseudomonas aeruginosa pathovar. Electrophoresis 1992, 13: 646–648.
Grothues D, Tümmler B New approaches in genome analysis by pulsed-field gel electrophoresis: application to the analysis ofPseudomonas species. Molecular Microbiology 1991, 5: 2763–2776.
Ziegler TR, Smith RJ, O'Dwyer ST, Demling RH, Wilmore W Increased intestinal permeability associated with infection in burn patients. Archives of Surgery 1988, 123: 1313–1319.
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Pradella, S., Pletschette, M., Mantey-Stiers, F. et al. Macrorestriction analysis ofPseudomonas aeruginosa in colonized burn patients. Eur. J. Clin. Microbiol. Infect. Dis. 13, 122–128 (1994). https://doi.org/10.1007/BF01982184
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DOI: https://doi.org/10.1007/BF01982184