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Acquired resistance to benomyl and some other systemic fungicides in a strain of Botrytis cinerea in cyclamen

Abstract

A benomyl-resistant strain (R) ofBotrytis cinerea was isolated from cyclamen that had been sprayed with relatively high doses of Benlate two weeks before. In vitro mycelial growth of this strain was less inhibited on PDA containing 1000 μg/ml benomyl (Benlate, 50% W.P.) than that of another, wild isolate ofB. cinerea from cyclamen on PDA with 0.5 μg/ml of the fungicide.

The R-strain was also resistant to methyl-thiophanate, furidazol and to a lesser extent to thiabendazole. Mycelial growth of 5 other isolates was much more inhibited by benomyl than by thiabendazole.

Resistance was retained for at least 20 weeks after repeated subculturing on fungicide-free agar.

Samenvatting

In een kwekerij, waar bespuiting met benomyl (Benlate, 50% W.P.) drie maal was toegepast ter bestrijding vanBotrytisrot in cyclamen, bleek de laatste bespuiting niet meer effectief. Integendeel, de ziekte breidde zich sneller uit dan onder normale omstandigheden het geval is. Uit bloemstelen van de aangetaste planten werd eenB. cinerea-stam (R) geïsoleerd, die zeer resistent bleek tegen benomyl. In vitro werd de groei van deze stam op aardappel-glucose-agar met 1000 μg/ml benomyl (Benlate 50% W.P.) minder geremd dan die van een willekeurigB. cinerea-isolaat van cyclamen op het medium met 0.5 μg/ml van het fungicide (Tabel 1, Fig. 1).

De R-stam bleek eveneens resistent tegen methyl-thiophanaat, furidazol en in mindere mate tegen thiabendazol (Tabel 2).

De myceliumgroei van vijf isolaten vanB. cinerea, verkregen van verschillende waardplanten, bleek in tegenstelling tot die van de R-stam juist sterker geremd te worden door benomyl dan door thiabendazol (Tabel 3).

De R-stam bleef gedurende tenminste 20 weken resistent na regelmatig overenten op voedingsbodems zonder het fungicide.

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Bollen, G.J., Scholten, G. Acquired resistance to benomyl and some other systemic fungicides in a strain of Botrytis cinerea in cyclamen. Netherlands Journal of Plant Pathology 77, 83–90 (1971). https://doi.org/10.1007/BF01981496

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  • DOI: https://doi.org/10.1007/BF01981496

Keywords

  • Mycelial Growth
  • Benomyl
  • Botrytis Cinerea
  • Thiabendazol
  • Systemic Fungicide